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Enhancing Teaching Effectiveness in Biochemistry Labs: Author Reflections and Improvement Strategies
Maurizio Costabile; Bradley Simpson; Jasmina Turkanovic; Bernard P. Hughes – Biochemistry and Molecular Biology Education, 2024
This article details the outcome of a joint reflective approach undertaken by the authors to identify common difficulties experienced by 2nd-year undergraduate Biochemistry students in laboratory classes. Difficulties experienced in laboratories can affect the development of hand skills, an understanding of how to correctly operate laboratory…
Descriptors: Biochemistry, Science Laboratories, Instructional Effectiveness, Undergraduate Study
Carmen Velez; Kristie Cheng; Sabrina Woodward; Noor Nazeer; Gabriela Nicole Hislop Gomez; Cody O. Crosby – Journal of Chemical Education, 2024
Many undergraduate chemistry and biochemistry students pursue careers in healthcare. Biomaterials, designed to harmonize with the complexity of the human body, have become increasingly significant in medicine as implants, drug delivery vehicles and tissue scaffolds. We believe it is essential that undergraduates are exposed to biomaterial…
Descriptors: Chemistry, Scientific Concepts, Medicine, College Science
Siddiqui, Khawar S.; Poljak, Anne; Ertan, Haluk; Bridge, Wallace – Biochemistry and Molecular Biology Education, 2022
Determination of enzyme activity is crucial for discovery, research, and development in life sciences. The activity of enzymes is routinely determined using spectrophotometric assays that measure rates of substrate consumption or product formation. Though colorimetric-based detection systems are simple, rapid, and economical to perform, the…
Descriptors: Biochemistry, Biological Sciences, College Science, Heat
Julie T. Millard; Ronald F. Peck; Tina M. Beachy; Victoria L. Hepburn – Journal of Chemical Education, 2023
An experiment for the upper-level biochemistry laboratory is described in which students isolate a wild yeast from environmental sources and characterize the strain for its potential in the brewing industry. In addition to providing valuable experience in important biochemical techniques, this study also illustrates key principles of…
Descriptors: Science Experiments, Laboratory Experiments, Biochemistry, Science Education
Kuzmanov, Aleksandra; Zwiesler-Vollick, Julie – Biochemistry and Molecular Biology Education, 2022
In this laboratory exercise, students have an opportunity to evaluate the potential endocrine disrupting abilities of environmental chemicals of their choice using human cell culture. Over the course of 9 weeks, students learn how to aseptically handle and manipulate cells, perform and analyze a cytotoxicity assay and an enzyme-linked…
Descriptors: College Science, Laboratory Experiments, Undergraduate Students, Science Instruction
Nicola´s Andre´s Saffioti; Carla Angelani; Jose´ Mari´a Delfino; Silvia Ferna´ndez Villamil; Gabriela Elena Go´mez; Irene Mangialavori; Mari´a Florencia Pignataro; Pablo Julio Schwarzbaum – Journal of Chemical Education, 2023
Metabolic Control Analysis (MCA) is a systems biology approach that measures the control of a metabolic step on the overall flux of a metabolic pathway. In undergraduate courses in biochemistry, learning of MCA can be encouraged by experimental activities demonstrating practical applications of the MCA theory. In this work, we present a laboratory…
Descriptors: Biochemistry, Metabolism, College Science, Undergraduate Students
Molecular Structure from Models to Mastery: An Inquiry-Based Study of Human Insulin-Degrading Enzyme
Michael A. Araujo; Alexandra A. Barrere; Selena-Rae Tirado; Candace E. Williams; Monica I. Strada; Benjamin J. Alper – Journal of Chemical Education, 2022
Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed…
Descriptors: Molecular Structure, Science Instruction, Science Laboratories, Science Experiments
Yan, Jingmin; Chen, Jiangao; Mao, Xuhu; Li, Qian – Biochemistry and Molecular Biology Education, 2023
As a universal and extensively adopted technique, enzyme-linked immunosorbent assay (ELISA) can be used to detect and quantify small molecules in many applications both clinical and analytical. However, generally, students experiment mechanically using commercial ELISA kits according to the instructions and eventually produce a standard curve to…
Descriptors: Undergraduate Students, Science Instruction, Biotechnology, Biochemistry
Mark A. Chrisman; Michael J. Goldcamp; Alexis N. Rhodes; Jared Riffle – Journal of Chemical Education, 2023
This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…
Descriptors: Science Laboratories, Science Experiments, Laboratory Experiments, Science Instruction
Garci´a-Ponce, A´ngel Luis; Torres-Vargas, Jose´ Antonio; Garci´a-Caballero, Melissa; Medina, Miguel A´ngel; Blanco-Lo´pez, A´ngel; Quesada, Ana R. – Journal of Chemical Education, 2021
Although many laboratory experiments are available to illustrate spectrophotometric or fluorometric methods, few of them introduce the use of luminometry to students. Bioluminescence, a subtype of chemiluminescence, is produced when an enzyme-catalyzed chemical reaction gives rise to light emission. Despite the advantages of bioluminescent…
Descriptors: Undergraduate Students, Laboratory Experiments, College Science, Biochemistry
Andrea C. Bardales; Quynh Vo; Dmitry M. Kolpashchikov – Journal of Chemical Education, 2024
It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which…
Descriptors: Active Learning, STEM Education, Scientific Concepts, Concept Formation
Crane, Julia; Page, Shallee T. – Biochemistry and Molecular Biology Education, 2022
We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published…
Descriptors: Undergraduate Students, College Science, Science Instruction, Scientific Research
Celia Todd; Carlos Melchor Ceballos; Monica C. So – Journal of Chemical Education, 2022
An integrated laboratory experience using metal--organic frameworks (MOFs), a nanoporous and crystalline extended solid material composed of metal cations and organic linkers, was developed and adopted for upper-division, major-level chemistry and biochemistry students. In this laboratory, students were guided through the facile solvothermal…
Descriptors: Water Pollution, Integrated Activities, Metallurgy, Laboratory Experiments
John C. Pinti; Elizabeth A. Kras; Akanksha Patel; Chloe L. Schrier; Christopher S. Stoj; Janet R. Morrow – Journal of Chemical Education, 2022
This undergraduate laboratory experiment is designed for first-year general chemistry students with the goal of introducing the brilliant colors and magnetic properties of transition metal complexes within a bioinorganic/cell biology context. In the first laboratory period, a coordination complex formed from ferric chloride and maltol is…
Descriptors: Magnets, Metallurgy, Undergraduate Study, Laboratory Experiments
Maqsood, Sehrish; Kilpatrick, Samuel M.; Truong, Chloe D.; Lefler, Scott R. – Journal of Chemical Education, 2021
During the COVID-19 pandemic, an at-home enzyme assay was developed for a biochemistry laboratory course at Arizona State University. The experiment was designed to use items that could be easily obtained and safe to use. The experiment focused on the analysis of salivary amylase using starch from food as a substrate, black tea as an inhibitor,…
Descriptors: Biochemistry, College Science, Hands on Science, Laboratory Experiments