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Enhancing Teaching Effectiveness in Biochemistry Labs: Author Reflections and Improvement Strategies
Maurizio Costabile; Bradley Simpson; Jasmina Turkanovic; Bernard P. Hughes – Biochemistry and Molecular Biology Education, 2024
This article details the outcome of a joint reflective approach undertaken by the authors to identify common difficulties experienced by 2nd-year undergraduate Biochemistry students in laboratory classes. Difficulties experienced in laboratories can affect the development of hand skills, an understanding of how to correctly operate laboratory…
Descriptors: Biochemistry, Science Laboratories, Instructional Effectiveness, Undergraduate Study
Van Wieren, Arie; Colen, Philip; Majumdar, Sudipta – Biochemistry and Molecular Biology Education, 2023
An understanding of structure-function relationships in proteins is essential for modern biochemical studies. The integration of common freely accessible bioinformatics tools available online with the knowledge of protein-engineering tools provide a fundamental understanding of the application of protein structure-function for biochemical…
Descriptors: Biochemistry, Science Instruction, Student Projects, Science Laboratories
Alexandra Taraboletti – Journal of Chemical Education, 2024
In response to the shifting modalities of laboratory-based education, particularly amidst the rise of virtual and at-home instruction, we introduce a novel hybrid laboratory module for undergraduate biochemistry and biology courses. Central to this innovative approach is the use of sourdough as a versatile and accessible model organism, enabling a…
Descriptors: College Science, Biochemistry, Biology, Science Education
Molecular Structure from Models to Mastery: An Inquiry-Based Study of Human Insulin-Degrading Enzyme
Michael A. Araujo; Alexandra A. Barrere; Selena-Rae Tirado; Candace E. Williams; Monica I. Strada; Benjamin J. Alper – Journal of Chemical Education, 2022
Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed…
Descriptors: Molecular Structure, Science Instruction, Science Laboratories, Science Experiments
Erasmus, Daniel J. – Biochemistry and Molecular Biology Education, 2021
Education in biochemistry teaching laboratories focus primarily on applying biochemical techniques to understanding human disease, biochemistry, and biotechnology. With anthropogenic climate change, there is a renewed interest in quantifying biodiversity, especially with the use of molecular-based approaches such as DNA barcoding. This 3-week…
Descriptors: Biochemistry, College Science, Undergraduate Students, Science Laboratories
Ioana Nicolau; Anca Paun; Codrut¸a C. Popescu; Niculina D. Ha?dade; Mihaela Matache – Journal of Chemical Education, 2023
This communication describes an updated experiment of peptide synthesis, involving determination of the loading capacity of a Fmoc-protected amino functionalized resin using UV-vis spectroscopy and a subsequent synthesis of a dipeptide in order to exemplify the experimental solid-phase synthesis procedure as an alternative technique in organic…
Descriptors: Organic Chemistry, Science Experiments, Spectroscopy, Biochemistry
Keira D. Naff; Jeri L. Tyler; Dima H. Hamza; Jonathan D. White – Journal of Chemical Education, 2023
Structure--activity relationships are foundational concepts in biochemistry and drug design. Despite their importance, it can be difficult to create and execute laboratory activities that allow students to directly observe the interactions between small-molecule drugs and their biomolecular targets. Here, we report an activity that investigates…
Descriptors: Genetics, Undergraduate Students, Undergraduate Study, College Science
Mark A. Chrisman; Michael J. Goldcamp; Alexis N. Rhodes; Jared Riffle – Journal of Chemical Education, 2023
This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…
Descriptors: Science Laboratories, Science Experiments, Laboratory Experiments, Science Instruction
Ramos-de-la-Peña, Ana Mayela; Mercado-Valenzo, Oscar Mario; Clorio-Carrillo, Jorge Abraham; López-Incio, Jazmin Dessiret; Monroy-Borrego, Andrea Gabriela; Marrero-Bretado, Mariana Monserrat; González-Valdez, José; Aguilar, Oscar – Biochemistry and Molecular Biology Education, 2021
A laboratory exercise for undergraduate advanced students of enzymology and biocatalysis is presented. Since enzyme assays can be quenched or continuous, this experiment compares the performance of two quenching agents for lactase, in a continuous setup. Enzymatic activity of [beta]-galactosidase ("Aspergillus oryzae") was determined…
Descriptors: Science Activities, Undergraduate Students, Advanced Courses, College Science
Andrea C. Bardales; Quynh Vo; Dmitry M. Kolpashchikov – Journal of Chemical Education, 2024
It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which…
Descriptors: Active Learning, STEM Education, Scientific Concepts, Concept Formation
Chatchadaporn Pinthong; Pimchai Chaiyen; Somchart Maenpuen; Pirom Chenprakhon – Journal of Chemical Education, 2022
This research developed learning activities for students to explore and connect the concepts of acid-base titration, equivalence points, pK[subscript a], and molar absorption coefficients. Polyphenolic compounds consisting of p-coumaric acid (CMA), caffeic acid (CFA), and 3,4,5-trihydroxycinnamic acid (3,4,5-THCA) were selected as study models.…
Descriptors: Active Learning, Inquiry, Science Laboratories, Chemistry
White, Rachel; Joyner, P. Matthew – Biochemistry and Molecular Biology Education, 2020
Flow cytometry has become an important tool in the life sciences and medical fields, yet there are often few opportunities for undergraduate students to receive training with this type of instrumentation as part of life science curricula at many colleges and universities. We describe a straightforward laboratory exercise designed for a…
Descriptors: Undergraduate Students, Biochemistry, College Science, Science Instruction
Keithley, Richard B.; Sullivan, Dylan T.; Dodd, John M., II; Iyer, Kavya V.; Sarisky, Catherine A.; Johann, Timothy W. – Journal of Chemical Education, 2021
Techniques and instrumentation that involve fluorescence are used in many careers related to biology and biochemistry. However, due to the expense of instrumentation, the theory and practice of fluorescence spectroscopy is commonly concentrated in instrumental analysis in most curricula, a class rarely required for biology and biochemistry majors.…
Descriptors: Chemistry, Science Instruction, College Science, Undergraduate Study
Valdivieso-Rivera, Fernando; Almeida, José Rafael; Proaño-Bolaños, Carolina – Biochemistry and Molecular Biology Education, 2022
Laboratory-based "practical" classes are an essential component in teaching molecular biology for undergraduate students. Universidad Regional Amazonica Ikiam is a higher education institution located in the Ecuadorian Amazon rainforest, a high biodiversity place, including amphibians. Based on this, we have established a practical…
Descriptors: Foreign Countries, Science Laboratories, College Science, Molecular Biology
Simões, Manuel; Malcata, F. Xavier – Journal of Chemical Education, 2020
A laboratory experiment is described in which students carry out immobilization of the enzyme invertase via entrapment in alginate (Ca-alginate) and, accordingly, estimate values for kinetic constants and mass transfer coefficients. Immobilization of enzymes, or confinement thereof to a defined space with retention of most catalytic activity, is…
Descriptors: Science Instruction, Science Laboratories, Scientific Concepts, College Science