The aim of this study is to compare high-intensity interval running exercises for physical education students in the field and the pool in terms of their effects on anaerobic power and blood lactate. 20 amateur male athletes that is student at physical education department participated in this study on a voluntary basis (Age: 22 ± 4 years, Height: 176 ± 8 cm, Weight: 72 ± 10 kg). The participants were divided into two groups, which were the pool group (n = 10) and the field group (n = 10). The athletes, who participated in the study, were administered a training program consisting of high-intensity interval running in the pool and the field during a period of eight weeks. Wingate test was administered to the participants and their lactate measurements were obtained before and after the 8-week training program. Data obtained were analyzed using the SPSS 22.0 package program. In order to determine the chronic effect of the training environment on anaerobic capacity and lactic acid activation, the difference between the findings obtained before and after the training was calculated. The independent sample-t test was performed to determine the difference between the groups. According to the results, aquatic exercises produced more positive gains on anaerobic power and lactic acid activation; however, there was no statistically significant difference between the groups (p > 0.05). In conclusion, it can be argued that the trainings in the pool could be included in the training programs as an alternative to field training due to the physical properties of water; and better results can be obtained to a certain extent.