This study aims to examine the relationship between teachers' attitudes toward artificial intelligence and their artificial intelligence literacy levels. In the present study, "General Attitude Toward Artificial Intelligence Scale" and "Artificial Intelligence Literacy Scale" were used. The study group consisted of 361 teachers working in public schools in Kartal, Pendik and Sultanbeyli districts of Istanbul province in Türkiye in the 2023-2024 academic year and selected by convenient sampling method. The findings obtained in this study showed that teachers' positive attitudes toward artificial intelligence were at a high level, while their negative attitudes were at a low level. The artificial intelligence literacy levels of the teachers were at a medium level. Teachers' positive and negative attitudes toward artificial intelligence and artificial intelligence literacy levels did not show significant differences according to their gender, professional seniority and education level. However, teachers' positive attitudes toward artificial intelligence and artificial intelligence literacy levels were significantly higher in teachers with graduate education than those with undergraduate education. Likewise, teachers' negative attitudes toward artificial intelligence were significantly higher in teachers with undergraduate education than those with graduate education. As a result of the correlation analysis, it was found that there was a positive, highlevel and significant relationship between teachers' positive attitudes toward artificial intelligence and their artificial intelligence literacy. On the other hand, there was a negative, moderate and significant relationship between teachers' negative attitudes toward artificial intelligence and their artificial intelligence literacy. As a result of the findings obtained from this study, some suggestions for researchers and practitioners are presented.