Relative adiposity (%Fat) was measured using a smartphone-based application in a convenience sample of adults aged 20-52 years (n = 32, 68.7% female, 84.3% White/Caucasian, 26.7 ± 3.5 kg/m2) across different body positions (Anterior versus Posterior) on consecutive days (Day 1 versus Day 2). A reference photo was obtained from the posterior view on Day 1 (POST[subscript Day1]), with an additional photo obtained from the anterior view (ANT[subscript Day1]). A third photo from the posterior view was obtained on Day 2 (POST[subscript Day2]). Potential differences were assessed using an adjusted alpha level (p < .025). ANT[subscript Day1] (26.44 ± 6.19%Fat) was not different than POST[subscript Day1] (26.84 ± 6.07%Fat, MAPE = 3.63%, p = 0.04) (ICC = 0.99, SEE = 1.1%Fat, p < .001). In addition, POST[subscript Day2] (26.65 ± 6.17%Fat) was not different than POST[subscript Day1] (MAPE = 3.30%, p = 0.31) (ICC = 0.99, SEE = 1.0%Fat, p < 0.001). Both ANT[subscript Day1] and POST[subscript Day2] were considered equal to POST[subscript Day1], suggesting %Fat can be consistently measured from a single digital image using a smartphone-based application in healthy young and middle-aged adults.