Biochemistry is a subject that is essential to understand the molecular basis of a disease, its diagnosis, and prognosis. However, study of biochemistry in dentistry at the undergraduate level is considered to be much teacher-oriented and the students show a lackluster attitude toward the subject. This questionnaire study is carried out to understand the perception of dental students in studying biochemistry and its relevance in dental practice. A pre-set questionnaire was sent to Bachelor of Dental Surgery students from first to final year and the responses were recorded. Although students are positive about liking biochemistry as a theory subject, still many are wary of the detailed study of complex reactions and metabolic pathways, which hampers the extensive study of the subject and its application in clinical practice.