The concepts of bacteriology are still poorly understood by students of the Department of Biology Education, Mulawarman University. Certain ethnic characteristics influence student learning processes and outcomes. A quasi-experiment was carried out at the Department of Biology Education, Mulawarman University in the even semester of the 2022/2023 academic year. The aim of the research was to map members of ethnic-based practicum groups in bacteriology practicum in an effort to empower understanding of bacteriology concepts. Ethnic-based practicum means that the practicum is carried out taking into account the ethnic diversity that exists in each group in the class. The research sample was class A and class B students who were taking a bacteriology practicum program. The research instruments were questionnaires and test questions. Data analysis techniques are percentage and covariance analysis. If there is a significant influence, then proceed with Least Significance analysis Difference. The results of data analysis show that there is a significant influence between the application of the product (a Project-Based Learning-based practicum guide) on understanding the concept of bacteriology as a result of development (p?0.00). The research sample was class A and class B students who were taking a bacteriology practicum program. For further research, practical material needs to be supplemented with other bacteriological materials, and involve more ethnic types.