Physical activity is linked to increased motivation and academic performance, yet the majority of research has focused on elementary and high school children. The current investigation evaluated this dynamic in college students (N = 209) by introducing a physical activity break at the mid-point of a videoed lecture and were divided into four conditions: control (no exercise), yoga, mild exercise, and moderate exercise. Students completed a motivation survey measure before the break and at the end of the lecture, followed by a quiz. Results showed that an intervention of moderate exercise and yoga were useful in increasing motivation and energy in college students. Interestingly, however, physical activity did not result in an increased retention of material. Future research is needed in a functioning classroom to evaluate the impact of exercise on academic motivation.