The aim of this study is to examine the validity, reliability, and responsiveness of the Turkish version of the Adolescent Health Promotion-Short Form (AHP-SF). This cross-sectional study was completed with 1483 students. Confirmatory factor analysis (CFA) supported the construct validity of the scale, demonstrating a good model fit with acceptable fit indices. Discriminant validity was established, revealing that lower AHP-SF scores were associated with an increased likelihood of unhealthy weight and chronic diseases. Reliability analyses revealed strong internal consistency, with Cronbach's alpha and McDonald's omega values indicating a high level of homogeneity among items. Test-retest reliability showed excellent stability, with no significant score differences between the two administrations. Differential item functioning (DIF) related to age suggests some items may perform differently for younger and older adolescents. However, most items showed negligible DIF, indicating reliable measurement of health-promoting behaviors across age groups. Item discrimination, difficulty, infit, and outfit results were within acceptable values. The AHP-SF is a valid and reliable tool for evaluating health-promoting behaviors of adolescents in Turkey. Nurses can assess health-promoting behaviors of adolescents in a short time by using this scale.