Wheat powdery mildew is an important fungal pathogen of wheat with an obligatory biotrophic lifestyle (a parasite that can only develop on a living host). We investigated the genetics of this host-pathogen interaction by using phenotyping and PCR assays to detect genes in both wheat and powdery mildew, which are known determinants of the outcome…

Despite the growing importance of agricultural microbiology at the applied, productive and ecological levels, undergraduate degrees in Agronomy generally do not feature much microbiological experimentation. To address this deficiency, we have designed an experimental programme that evaluates the potential of plant growth-promoting rhizobacteria…

A community-engaged learning experiment was developed for an introductory analytical chemistry course at the University of Toronto Scarborough in collaboration with Campus Farm. The experiment introduced students to the application of analytical techniques to studying environmental samples. More specifically, students investigated the potential of…

Universities play an important role in society and in providing solutions for its problems. Environmental pollution is one of the most serious problems facing the world today. Thus, universities are increasingly including subjects related to the environment and its conservation both in their educational and research programmes. Generally,…

This practical paper describes a novel fluorescence imaging experiment to study the three processes of photochemistry, fluorescence and thermal energy dissipation, which compete during the dissipation of excitation energy in photosynthesis. The technique represents a non-invasive tool for revealing and understanding the spatial heterogeneity in…

The importance of mathematical literacy in any scientific career is widely recognized. However, various studies report lack of numeracy and mathematical literacy in students from various countries. In the present work, we present a detailed study of the mathematical literacy of Spanish undergraduate students of Biology enrolled in a Plant…

Students often prefer to study animals rather than plants, because they see plants as passive, less interesting organisms. This paper proposes a simple hands-on laboratory exercise for high-school students (grade 12) to arouse their interest in learning about plants and to demonstrate to them that plants are active organisms capable of defending…

In this experiment, the extract from annatto seeds was encapsulated in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) particles by the emulsion-solvent evaporation method. The particles were washed and centrifuged to remove excess stabilizer and then freeze-dried. The main compound of annatto seeds, bixin, has antioxidant properties as well…

An HPLC undergraduate experiment on the analysis of traditional Chinese medicine (TCM) has been developed. Two commonly used herbs ("glycyrrhizae radix" and "cinnamomi ramulus") are studied. Glycyrrhizin, cinnamic acid, and cinnamaldehyde are chosen as markers for the herbs. The dried herbs in their natural state and a TCM…

The purpose of this study is to prepare inquiry based experimental activities on the photosynthesis, thought to be a very difficult subject by pupils, and to determine the pupil's ideas towards this method. This study was made with 24 pupils from Grade 3 at Ataturk Anatolian High school in Turkey. As data gathering material; seven inquiry…

Demonstrates how leaf slices provide an inexpensive material for illustrating several fundamental points about the biochemistry of photosynthesis and respiration. Presents experiments that illustrate the effects of photon flux density and herbicides and carbon dioxide concentration. (DDR)

This paper describes some simple and rapid techniques for examining the growth responses of fungal hyphae cultivated on environmental gradients. The creation of such gradients using agar-based growth media in petri dishes is explained, along with recommendations for quantitative macroscopic and microscopic measurements. The intention is to provide…

In this paper we present a simple set-up that illustrates the mechanism of sap ascent in plants and demonstrates that it can easily draw water up to heights of a few meters. The set-up consists of a tube with the lower end submerged in water and the upper one connected to a filter supported by a standard filter-holder. The evaporation of water…