Knowledge and stewardship are essential factors in developing perceptions of the environment. This research determines if gaps in environmental knowledge and concern exist between genders as well as students majoring in science and those seeking non-science degrees. Approximately 1,229 first-year university students participated in this study. Environmental concern is measured with the New Ecological Paradigm and the Environmental Concern Scale. Total Environmental Knowledge is measured by a scale of 24 academic questions based on environmental sciences and behaviors. Outcomes of the study determined the strength of interactions between multiple factors such as gender, race, residential status and college major that potentially impact environmental knowledge, actions and behaviors. Findings suggest that females and science majors express higher levels of environmental concern.