Objective: Assess nutrition knowledge of Division I college athletes. Participants: 128 student-athletes (n¼ 70 female) from eight sports completed the survey in June 2018. Methods: The survey by Calella et al (2017) was used to assess both general and sport nutrition knowledge. Results: Cases with more than 20% of responses missing were excluded (n ¼ 3). Overall average score was 57.6% ± 18.6%. Females scored significantly (p < 0.001) better than the males (66.5% ± 16.4% versus 46.2% ± 14.7%). Participants were divided into revenue (football, ice hockey, male's basketball, women's basketball; n ¼ 63) and non-revenue sports (field hockey, golf, rowing, soccer; n ¼ 62) to address differences in knowledge between sports with greater versus lesser nutrition resource access. Revenue sports scored significantly (p < 0.001) worse than non-revenue sports (45.7% ± 15.2% versus 69.7% ± 13.1%). Conclusions: Athletes appear to have low nutrition knowledge, putting them at risk for inappropriate dietary choices that could decrease ability to optimally perform and increase risk of injury