The complexity of RNA cannot be fully expressed with the canonical A, C, G, and U alphabet. To date, over 170 distinct chemical modifications to RNA have been discovered in living systems. RNA modifications can profoundly impact the cellular outcomes of messenger RNAs (mRNAs), transfer and ribosomal RNAs, and noncoding RNAs. Additionally, aberrant…

The rapid growth of online education has created opportunities to integrate multimedia learning tools into complex scientific disciplines like food biotechnology. This study aimed to develop and evaluate supplementary online course modules on gene expression analysis, protein engineering tools, and fermentation genomics for undergraduate food…

The Genomics Education Partnership (GEP; thegep.org) is a collaboration of more than 260 faculty from over 200 colleges and universities across the continental United States and Puerto Rico, all of whom are engaged in bringing Course-based Undergraduate Research Experiences (CUREs) centered in genomics and bioinformatics to their students. The…

Forward genetics involves the identification of an unknown DNA sequence (genotype) associated with the expression of that sequence (phenotype). This is often done by generating variants (mutants) of that feature. One of the most practical methods is transposon mutagenesis, which facilitates the genotype-phenotype association through a sequence…

Wheat powdery mildew is an important fungal pathogen of wheat with an obligatory biotrophic lifestyle (a parasite that can only develop on a living host). We investigated the genetics of this host-pathogen interaction by using phenotyping and PCR assays to detect genes in both wheat and powdery mildew, which are known determinants of the outcome…

Undergraduate students of a molecular cell biology laboratory class learn basic recombinant DNA (rDNA) technology by engaging in a Genomic Library Construction Project. Significantly, in this lab project students learn experimental design strategy. The challenge for students is to determine the strategy that they can take to efficiently screen for…

This dissertation consists of two distinct bodies of work. The first leverages a unique aspect of fire ant biology to investigate a potential role for a supergene in genetic assimilation. A supergene refers to a chromosome structural rearrangement, such as an inversion, that regulates the production of an alternative phenotype within a species. In…

Apurinic/apyrimidinic (AP or abasic) sites are deoxyribonucleic acid (DNA) lesions that result from the loss of a nucleobase by hydrolysis of the "N"-glycosyl bond. AP sites are the most frequent lesions in cells and are caused by environmental and cancer therapeutic genotoxins. Herein, the bicinchoninic colorimetric assay, typically…

Graduates in biology or genetics learn Mendel's laws and Hardy-Weinberg equilibrium as students, and they know, use, and sometimes teach these concepts. However, it is unusual to learn about stochastic processes during the graduate studies of these topics, although the applications of Markov chains in the fields of genetics or biology are…

Course-based undergraduate research experiences (CUREs) provide students with valuable opportunities to engage in research in a classroom setting, expanding access to research opportunities for undergraduates, fostering inclusive research and learning environments, and bridging the gap between the research and education communities. While…

Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from…

The present study has two main goals. First, it aims to examine the analogies an instructor uses about "DNA conservation across generations" in a university-level General Biology course. Second, it aims to examine the analogies used in those lessons according to Thiele and Treagust's framework. A qualitative case study design is adopted…

The field of DNA nanotechnology has grown rapidly in the past decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their…

Education in biochemistry teaching laboratories focus primarily on applying biochemical techniques to understanding human disease, biochemistry, and biotechnology. With anthropogenic climate change, there is a renewed interest in quantifying biodiversity, especially with the use of molecular-based approaches such as DNA barcoding. This 3-week…

Multiple studies have shown that testing contributes to learning at all educational levels. In this observational classroom study, we report the use of a learning tool developed for a Genetics and Molecular Biology course at the college level. An interactive set of practice exams that included 136 multiple choice questions (MCQ) or matching…