The aim of this study was to develop molecular genetics inquiry programs using the "eyes absent" gene of "Drosophila melanogaster." The program was composed of various molecular genetics experiments, including mutation observation, cross-breeding, searching for genetic information in web databases, gDNA extraction, and PCR.…

Wheat powdery mildew is an important fungal pathogen of wheat with an obligatory biotrophic lifestyle (a parasite that can only develop on a living host). We investigated the genetics of this host-pathogen interaction by using phenotyping and PCR assays to detect genes in both wheat and powdery mildew, which are known determinants of the outcome…

Undergraduate students of a molecular cell biology laboratory class learn basic recombinant DNA (rDNA) technology by engaging in a Genomic Library Construction Project. Significantly, in this lab project students learn experimental design strategy. The challenge for students is to determine the strategy that they can take to efficiently screen for…

Structure--activity relationships are foundational concepts in biochemistry and drug design. Despite their importance, it can be difficult to create and execute laboratory activities that allow students to directly observe the interactions between small-molecule drugs and their biomolecular targets. Here, we report an activity that investigates…

It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which…

The study focused on the development of a website that can be used in various fields of science in Junior High School. Particularly, topics that were utilized as part of the re-search report include chromosomes, DNA, and genes for grade 9 learners. This exploratory case study explored learners' engagement and performance on a developed virtual…

We used a novel approach that combined direct PCR of plant tissue and SRAP DNA fingerprinting to seek to identify the crosses made in "Brassica rapa" used in the Wisconsin Fast Plants. Our goal was to develop a rapid method for students to use in a biology, botany, or genetics class to identify the parental types (purple stem, non-purple…

The Förster resonance energy transfer (FRET) melting assay intends to evaluate the unfolding, denaturation process of DNA secondary structures, and its stabilization using compounds known as DNA binders, some of which are highly specific for G-quadruplex DNAs versus duplex DNAs. First, students determined the melting temperature (T[subscript m])…

Genetics is known to be one of the most challenging subjects in biology education because of its abstract concepts and processes. Therefore, hands-on experiments in authentic learning environments are supposed to increase comprehensibility and provide otherwise unavailable experiences to students. We applied a hands-on module in an out-of-school…

Most undergraduate students do not have practical experience working with model organisms despite having theoretical knowledge of their importance. This practical was developed to offer students experience working with three species of nematode, including "Caenorhabditis elegans." In the first task, students prepare slides of three…

Nowadays, novel Biochemistry lab techniques are being introduced at a very fast pace in scientific research. This requires development of new labs for undergraduate Biochemistry courses to equip the students with up-to-date techniques. However, the time limit of Biochemistry labs for undergraduate students represents a major obstacle. This article…

Research and education are two areas often compartmentalised or separated by an abyss, linked in this study by a service-learning methodology, the Small World Initiative project. The programme instruction starts at the university, reaches secondary school and returns to university to complete the research, challenging young students to discover…

Genetics is an important basic course in the field of life science, and the cultivation of students' practical ability should be emphasized in the experimental teaching of genetics. How to let students have more time to participate in the experimental practice within the limited class hours? Our genetics experimental teaching team has developed…

Next generation sequencing has revolutionized molecular biology and has provided a mechanism for rapid DNA and RNA sequence analysis. Yet, there are few resources to introduce next generation sequencing into the undergraduate biochemistry and molecular biology curriculum. Herein, we describe the design, execution, and assessment of a four-week…

Proteins must interact with a variety of other cellular components to properly perform their functions. We have developed a series of five experiments based on the robust bacterial single-stranded DNA binding protein (SSB) to characterize both known and unknown protein-protein and protein-DNA interactions. Students work in groups to generate and…