Guided notes are an instructional strategy used to help students retain facts, concepts, and skills. Evidence for the effectiveness of guided notes with school-aged children diagnosed with high incidence disabilities is well documented, but research with postsecondary participants has had mixed results, and there has been little to no research on the effects of guided notes in online learning environments. The present study sought to examine the effects of guided notes on the retention of facts in high achieving online graduate students. Results indicate statistical significance (p < 0.01) in performance with a medium effect size, and students preferred guided notes to assist with note taking during lectures.