In the past decades, zoos have increasingly developed into conservation and education centers and today make an important contribution to environmental education. In this context, this study investigated which factors influence attitudes towards species conservation. The variables examined were gender, age, the number of visits to zoos in the last 12 months, perception of zoos, interest in animals and the country where the survey was conducted. A total of 3347 participants in seven different countries were surveyed. In the hierarchical multiple regression, it was found that all the variables examined were significant influencing factors with exception of gender. A mediator analysis provided evidence that the number of visits to zoos, in addition to the direct effect on attitudes towards species conservation, also has a relevant indirect effect with interest in animals as mediators. Significant differences in attitudes towards species conservation were found between some of the countries studied, but only with a small effect sizes.