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Showing 1 to 15 of 515 results Save | Export
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Alison Bates; Kathryn M. Williams; Ann E. Hagerman – Biochemistry and Molecular Biology Education, 2025
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…
Descriptors: Undergraduate Students, Science Instruction, Laboratory Experiments, Kinetics
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Bolduc, Kathryn C.; McCollough, Shannon L.; Stoeckman, Angela K. – Biochemistry and Molecular Biology Education, 2023
Undergraduate laboratory courses are vital for both enhancing student learning and preparing students for their future careers. Despite their importance, laboratory courses are often met with a lack of enthusiasm from students. For pre-health students specifically, laboratory courses are commonly seen as part of a check-list that needs to be…
Descriptors: Biochemistry, Science Laboratories, Laboratory Training, Majors (Students)
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Naoto Shirasu; Shin'ichiro Yasunaga – Biochemistry and Molecular Biology Education, 2024
Here, we propose a laboratory exercise to quickly determine single nucleotide polymorphisms (SNPs) in human "alcohol dehydrogenase 1B (ADH1B)" and "aldehyde dehydrogenase 2 (ALDH2)" genes involved in alcohol metabolism. In this exercise, two different genotyping methods based on polymerase chain reaction (PCR), namely…
Descriptors: Genetics, Biochemistry, Students, Science Instruction
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Maurizio Costabile; Bradley Simpson; Jasmina Turkanovic; Bernard P. Hughes – Biochemistry and Molecular Biology Education, 2024
This article details the outcome of a joint reflective approach undertaken by the authors to identify common difficulties experienced by 2nd-year undergraduate Biochemistry students in laboratory classes. Difficulties experienced in laboratories can affect the development of hand skills, an understanding of how to correctly operate laboratory…
Descriptors: Biochemistry, Science Laboratories, Instructional Effectiveness, Undergraduate Study
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Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna – Biochemistry and Molecular Biology Education, 2021
The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In…
Descriptors: Biochemistry, Cytology, Metabolism, Science Laboratories
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Louise Castro de Jesus; Sophia Lincoln Cardoso de Azevedo; Rafaela Moraes Pereira de Sousa; Ana Beatriz Matos; Salim Kanaan; José Carlos Pelielo de Mattos; Lidia Maria Amorim – Journal of Biological Education, 2025
Isoenzymes are variant forms of the same enzyme activity that exhibits different tissue distribution. When cells are damaged, intracellular content leaks into the blood stream, making them potential biochemical markers. Undergraduate health sciences students are expected to interpret laboratory findings of serum levels of biochemical markers and…
Descriptors: Science Laboratories, Biochemistry, Health Sciences, Allied Health Occupations Education
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Nicola´s Andre´s Saffioti; Carla Angelani; Jose´ Mari´a Delfino; Silvia Ferna´ndez Villamil; Gabriela Elena Go´mez; Irene Mangialavori; Mari´a Florencia Pignataro; Pablo Julio Schwarzbaum – Journal of Chemical Education, 2023
Metabolic Control Analysis (MCA) is a systems biology approach that measures the control of a metabolic step on the overall flux of a metabolic pathway. In undergraduate courses in biochemistry, learning of MCA can be encouraged by experimental activities demonstrating practical applications of the MCA theory. In this work, we present a laboratory…
Descriptors: Biochemistry, Metabolism, College Science, Undergraduate Students
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Andrea C. Bardales; Quynh Vo; Dmitry M. Kolpashchikov – Journal of Chemical Education, 2024
It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which…
Descriptors: Active Learning, STEM Education, Scientific Concepts, Concept Formation
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Matthew R. Anderson; Cammi J. Dargatz; Tuhina Banerjee; Natasha M. DeVore – Biochemistry and Molecular Biology Education, 2024
Green fluorescent protein has long been a favorite protein for demonstrating protein purification in the biochemistry lab course. The protein's vivid green color helps demonstrate to students the concept(s) behind affinity or ion exchange chromatography. We designed a series of introduction to biochemistry labs utilizing a thermostable green…
Descriptors: Science Education, Biochemistry, Laboratory Experiments, Color
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Mark A. Chrisman; Michael J. Goldcamp; Alexis N. Rhodes; Jared Riffle – Journal of Chemical Education, 2023
This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel­(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…
Descriptors: Science Laboratories, Science Experiments, Laboratory Experiments, Science Instruction
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Michael A. Araujo; Alexandra A. Barrere; Selena-Rae Tirado; Candace E. Williams; Monica I. Strada; Benjamin J. Alper – Journal of Chemical Education, 2022
Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed…
Descriptors: Molecular Structure, Science Instruction, Science Laboratories, Science Experiments
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Katherine R. McCance; Ana Maria Topliceanu; Darlene Echeverria; Shana L. McAlexander; Margaret R. Blanchard; Richard A. Venditti – Journal of Chemical Education, 2023
As e-commerce increases, the demand for packaging materials and the potential for generating waste and negative environmental impacts also rise. Packing peanuts are a type of plastic packaging material that are used to protect goods during the shipping process. Petroleum-based plastics are common packaging materials due to their low cost, light…
Descriptors: Chemistry, Plastics, Recycling, Conservation (Environment)
Muhammad Saefi; Widi Cahya Adi; Amining Rahmasiwi; Hidayati Maghfiroh; M. Eval Setiawan; Miza Nina Adlini; Syarif Rizalia – Journal of Biological Education Indonesia (Jurnal Pendidikan Biologi Indonesia), 2023
Laboratory course activities often focus on "cookbook style," so their activities have not yet integrated with scientific thinking as an essential aspect. In this research, the laboratory experience of microbial analysis techniques was redesigned using alternative inquiry to involve students in isolating and characterising yeast to make…
Descriptors: Laboratory Experiments, Teaching Methods, Food, Cooking Instruction
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Kater?ina Trc?kova´; Hana Tkac?i´kova´; Roman Mars?a´lek – Journal of Chemical Education, 2023
The purpose of this article is to describe the possibilities of using worksheets in the teaching of lipids and proteins. The worksheet includes a concept map, suggestions for three safe experiments that can be performed with available household chemicals, and observation results. The worksheets were implemented into action research in two classes…
Descriptors: Laboratory Experiments, Laboratory Safety, Worksheets, Science Instruction
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Lagares, Antonio, Jr.; Garavaglia, Matías Javier; Robledo, Natalia Belén; Valverde, Claudio; Goñi, Sandra Elizabeth; Lozano, Mario Enrique – Journal of Biological Education, 2022
Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to…
Descriptors: Metabolism, Biochemistry, Visualization, Laboratory Experiments
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