Adsorption of methylene blue and malachite green was carried out using "Luffa cylindrica" sponges as adsorbing material. We provide a very illustrative exercise about the use of common natural materials requiring little preparation to solve environmental tasks in a routine laboratory situation such as using dyes. In addition, the…

To inspire upper-division undergraduate students to gain a deeper understanding of biorefinery, an experiment related to biofuels and lignin-based sunscreen products from corncob was performed in multiple offerings of a summer-semester class. Specifically, corncobs were pretreated with 1% H[subscript 2]SO[subscript 4] to obtain hemicellulose…

A new laboratory experiment investigating the ternary phase equilibrium of water-propan-2-ol-sodium chloride with one-, two-, and three-phase regions has been developed. Observations of the type and number of phases of a series of mixtures of the three components were made, and the results are then used in a systematic way to more finely choose…

An experiment involving a moisture-sensitive synthesis and spectroscopic characterization suitable for the advanced inorganic laboratory course is presented. The acid-base complex ammonia borane (AB) is synthesized from the heterogeneous reaction between sodium borohydride and finely ground ammonium sulfate in tetrahydrofuran solvent. AB is…

The different reactivities between 1°, 2°, and 3° butyl chlorides by the unimolecular nucleophilic substitution (S[subscript N]1) mechanism were easily observed at 60 °C by a solvolysis reaction in ethanol with ether formation. The hydrogen ion from the byproduct HCl reacted with an acid--base indicator such as methyl red to induce a color change.…

An experiment for the upper-division undergraduate biochemistry laboratory is described in which students investigate the influence of genetic variations of cytochrome P450 1A2 on drug metabolism, using caffeine as a model compound. Saliva samples from human subjects are characterized for a single-nucleotide polymorphism in the CYP1A2 gene…

Reported herein is a multidisciplinary laboratory experiment for advanced undergraduate students that includes elements of material synthesis, in the synthesis of cyclodextrin-containing metal-organic frameworks (CD-MOFs), and environmental chemistry, in the use of these MOFs for pollutant removal. This multiday laboratory experiment starts with…

Disrupting a gene to determine its effect on an organism's phenotype is an indispensable tool in molecular biology. Such techniques are critical for understanding how a gene product contributes to the development and cellular identity of organisms. The explosion of genomic sequencing technologies combined with recent advances in genome-editing…

This is the story of how an enterprising high school student came to my lab one afternoon, asking if there were any way that he could gain "hands-on" lab experience by working with me. While I had some doubts about allowing him to work in an area with an expensive 150-mW focused laser beam, I eventually said yes. I was well aware that a…

A laboratory experiment often performed in an introductory electricity and magnetism course involves the mapping of equipotential lines on a conductive sheet between two objects at different potentials. In this article, we describe how we have expanded this experiment so that it can be used to illustrate the electrostatic properties of conductors.…

Multidisciplinary lab experiments combining microfluidics, nanoparticle synthesis, and characterization are presented. These experiments rely on the implementation of affordable yet efficient microfluidic setups based on perfluoroalkoxyalkane (PFA) capillary coils and standard HPLC connectors in upper undergraduate chemistry laboratories.…

Ubiquitin is essential for eukaryotic life and varies in only 3 amino acid positions between yeast and humans. However, recent deep sequencing studies indicate that ubiquitin is highly tolerant to single mutations. We hypothesized that this tolerance would be reduced by chemically induced physiologic perturbations. To test this hypothesis, a class…

Mass spectrometry is frequently introduced to undergraduate students as an instrument for both qualitative and quantitative chemical analysis. One of the most common uses of mass spectrometry (MS) is to deduce or confirm a compound's chemical formula, by relying on high-resolution accurate-mass measurements. However, like all forms of instrumental…

Inquiry-based learning can be an effective approach when students are provided with sufficient guidance. However, there is an ongoing debate among practitioners about the nature, type, and level of such guidance. This study investigated the effects on middle school students' (N = 116) conceptual knowledge and inquiry skills acquisition of…

The glowing discharges of plasma obtained by introducing direct current high voltages between two electrodes in a chamber that contains a low pressure gas produces a spectacular effect that makes it attractive to be used as a laboratory experiment for students. Setting up this experiment is however costly and has crucial safety issues to be…