Discusses materials needed, procedures used, and typical results obtained for experiments designed to make a numerical stepwise study of the oxygenation of hemoglobin, myoglobin, and other oxygen carriers. (JN)

A three-period experiment was developed to help students visualize asymmetric distribution of proteins within membranes. It includes: (1) isolating erythrocyte membranes; (2) differential labeling of intact erythrocytes and isolated erythrocyte membranes with an impermeable fluorescent dye; and (3) separating proteins by polyacrylamide gel…

Uses the subject of enzymes to illustrate the topical approach to teaching biochemistry at the secondary level. Includes the procedures for three experiments dealing with various aspects of enzymes. (JN)

Describes quick and easy experiments that yield quantitative information on a variety of levels, emphasize the concept of experimental controls, and integrate the experimental with the theoretical using the organic phosphates as the experimental system. Background information, list of materials needed, and procedures used are included. (JN)

Describes an undergraduate enzyme kinetics experiment which uses bovine erythrocyte carbonic anhydrase, a very stable enzyme commercially available in lyophilized form. Includes background information, reactions involved, procedures used, and the calculation of typical results obtained. (JN)

Describes the nature and capabilities of methods used to characterize protein and peptide structure, indicating that they have undergone changes which have improved the speed, reliability, and applicability of the process. Also indicates that high-performance liquid chromatography and gel electrophoresis have made purifying proteins and peptides a…

Describes an experiment to characterize the enzyme polyphenol oxidase. Includes Purpose, Reagents and Equipment, Procedure, Extraction, Treatment of Data, and Discussion. (MA)

Described is the use of fluorometry in an experiment where the fluorescence of riboflavin is specifically quenched when titrated with riboflavin-binding protein. The amount of riboflavin, required by all organisms, in an unknown solution and the dissociation constant of the vitamin-protein complex can be determined using this method. (KR)

Describes two frequently used DNA sequencing methods: Sander's enzymatic dideoxy method and Maxam and Gilbert's chemical sequencing method. Indicates that studying these methods provides students with knowledge of the chemical structure of DNA and how DNA sequence data are obtained. (JN)

Background information, procedures, and results are provided for an experiment in which students: (1) construct an immobilized penicillinase electrode; (2) evaluate electron response as a function of penicillin concentration; and (3) study effects of solvent compositions, ionic strength, and equilibrium time upon electrode response. (JN)

Described is a laboratory procedure which uses the small intestines of mice as models for the transport of glucose and other solutes. Demonstrations are suitable for either introductory or advanced physiology courses. (RE)

Describes an exercise that illustrates the synthesis of an amino acid and Michael type condensation. The exercise can be used with college students of chemistry or biochemistry. (HM)

Biomimetic chemistry is concerned with the synthesis of small, molecular weight molecules which mimic the properties of metal-containing sites within certain biologically significant species. A series of experiments for an advanced undergraduate laboratory is described as a way to introduce this area into the chemistry curriculum. (JN)

Background information, experimental approach, materials needed, procedures used, and typical results obtained are provided for genetic engineering experiments. The course in which these experiments are performed is also described. (JN)

Describes an experiment which allows students to identify the nature of the time-course for chymotrypsin and to obtain mechanistic information on the two kinetic phases in terms of their concentration dependence. Also describes the computer simulation (available from the author) used in the experiment. (JN)