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Direct linkAlison Bates; Kathryn M. Williams; Ann E. Hagerman – Biochemistry and Molecular Biology Education, 2025
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…
Descriptors: Undergraduate Students, Science Instruction, Laboratory Experiments, Kinetics
Andrew J. Tonsager; Laurie A. Stargell – Biochemistry and Molecular Biology Education, 2025
CRISPR-Cas9 technology is an established, powerful tool for genome editing through the ability to target specific DNA sequences of interest for introduction of desired genetic modifications. CRISPR-Cas9 is utilized for a variety of purposes, ranging from a research molecular biology tool to treatment for human diseases. Due to its prominence…
Descriptors: Undergraduate Students, Student Research, Scientific Research, Genetics
Ângelo Luís; Fernanda Domingues – Biochemistry and Molecular Biology Education, 2025
Bacteria have developed a cell-to-cell communication system called quorum sensing (QS), allowing them to regulate group behavior and synchronize the expression of virulence factors, responsible for increasing their infection capacity and resistance to antimicrobials. Although the control of microbial infections through the inhibition of microbial…
Descriptors: Inhibition, Evaluation Methods, Science Experiments, Microbiology
Maurye, Praveen; Basu, Arpita; Jana, Chayna; Devi, Manoharmayum Shaya; Kumari, Kavita; Naskar, Malay – Biochemistry and Molecular Biology Education, 2022
Gel electrophoresis (GE) is the most preferred and adapted technique for the separation and identification of biological molecules like proteins/peptides and nucleic acids from diverse types of organisms. All over the world, researchers, educators, and students aspiring to work in biochemistry and molecular biology disciplines use the…
Descriptors: High School Students, Biochemistry, Molecular Biology, Laboratory Procedures
Bo Ding; Ju Wang; Yaxin Mao; Tian Meng; Yumei Luo; Xiaomei Xu; Zhanqing Lv; Mian Zhou; Qiuying Yang – Biochemistry and Molecular Biology Education, 2024
Fluorescence-related experimental techniques play an important role in biochemistry, molecular biology, and cell biology. However, fluorescence-related experiments are rarely included in the laboratory courses of most Chinese universities. This is mainly due to the conflict between large class size (50-60 students in one room) and funding/space…
Descriptors: Science Education, Science Laboratories, Laboratory Training, Laboratory Procedures
Harshitha, Ravikumar; Arunraj, Duraipandian Rex – Biochemistry and Molecular Biology Education, 2021
Real-time quantitative PCR is a technique used to monitor the PCR reaction in real time. RT-qPCR is broadly classified into two types based on its purpose: absolute and relative quantification. Absolute quantification is used in a wide array of fields such as microbiology, food technology, and biotechnology to quantify the microbiological…
Descriptors: Laboratory Procedures, Laboratory Experiments, College Science, Molecular Biology
Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna – Biochemistry and Molecular Biology Education, 2021
The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In…
Descriptors: Biochemistry, Cytology, Metabolism, Science Laboratories
Beheshtian, Cheyenne; Garcia, Vanessa Elena; Zhu-Hui Ng, Tiffany; Alkhatib, Sarah; Quang, Erica; Cho, Kira Jinju; Nguyen, Timothy Duy; Le, Dustin Nguyen; Kadandale, Pavan – Biochemistry and Molecular Biology Education, 2023
A large body of literature has established the benefits of undergraduate research experiences via the traditional apprenticeship model. More recently, several studies have shown that many of these benefits can be recapitulated in course-based undergraduate research experiences (CUREs) that are more scalable and easier for students to participate…
Descriptors: Undergraduate Students, Student Research, Research Training, Laboratory Experiments
Angelani, Carla R.; Carabias, Pablo; Cruz, Karen M.; Delfino, José M.; Sautu, Marilina; Espelt, María V.; Ferreira-Gomes, Mariela S.; Gómez, Gabriela E.; Mangialavori, Irene C.; Manzi, Malena; Pignataro, María F.; Saffioti, Nicolás A.; Salvatierra Fréchou, Damiana M.; Santos, Javier; Schwarzbaum, Pablo J. – Biochemistry and Molecular Biology Education, 2018
Metabolic control analysis (MCA) is a promising approach in biochemistry aimed at understanding processes in a quantitative fashion. Here the contribution of enzymes and transporters to the control of a given pathway flux and metabolite concentrations is determined and expressed quantitatively by means of numerical coefficients. Metabolic flux can…
Descriptors: Metabolism, Biochemistry, Cytology, Laboratory Procedures
Pufall, Miles A.; Wilson, Anne M. – Biochemistry and Molecular Biology Education, 2020
Providing students with training in advanced laboratory skills is an essential part of scientific education. At the same time, engaging students in research is becoming equally important. Classroom-based undergraduate research experiences (CUREs) have emerged to fill this need, and can take many forms. In this article we describe reengineering an…
Descriptors: Undergraduate Students, Student Research, Student Experience, Institutional Cooperation
Ortiz Lechuga, Eugenia Guadalupe; Tovar Herrera, Omar Eduardo; Arévalo Niño, Katiushka – Biochemistry and Molecular Biology Education, 2019
In this work, we present the results of an undergrad student from the perspective of its first approach as a principal researcher in a project. In order to gain practical experience, one of the options for students that have interest in pursuing a postgraduate program corresponds to a research stay in a laboratory of their selected field…
Descriptors: Undergraduate Students, College Science, Biotechnology, Biochemistry
Quilis, Inma; Igual, J. Carlos – Biochemistry and Molecular Biology Education, 2018
This article describes a laboratory exercise designed for undergraduate students in the subject of "Regulation of cell proliferation" which allows the students to carry out a research experiment in an important field such as cell cycle control, and to be introduced to a widely used technique in molecular biology laboratories such as the…
Descriptors: Cytology, Biochemistry, Laboratory Experiments, Molecular Biology
Marchioretto, Mira K.; Horton, Jacqueline T.; Berstler, Calvin A.; Humphries, Joseph B.; Koloditch, Isaac J.; Voss, Samuel D.; de La Harpe, Kimberly; Hicks, Barry W.; Jefferies, Latisha R. – Biochemistry and Molecular Biology Education, 2018
Förster resonance energy transfer (FRET) is the basis for many techniques used in biomedical research. Due to its wide use in molecular sensing, FRET is commonly introduced in many biology, chemistry, and physics courses. While FRET is of great importance in the biophysical sciences, the complexity and difficulty of constructing FRET experiments…
Descriptors: Undergraduate Students, Biomedicine, Laboratory Procedures, Laboratory Experiments
González, Andrés; Fillat, María F. – Biochemistry and Molecular Biology Education, 2018
Recombinant protein expression and site-directed mutagenesis of target genes have demonstrated an increasing importance in the fields of molecular biology, biochemistry, biotechnology, and medicine. By using the flavodoxin of the model cyanobacterium "Anabaena" sp. PCC 7120 as a laboratory tool, we designed a comprehensive laboratory…
Descriptors: Undergraduate Students, Genetics, Molecular Biology, Science Laboratories
Yap, Wei Hsum; Teoh, Ming Li; Tang, Yin Quan; Goh, Bey-Hing – Biochemistry and Molecular Biology Education, 2021
This study presents an evaluation of integrating virtual laboratory simulations in assessment design of a biotechnology course at Taylor's University in Malaysia before, during and post-COVID recovery phases. The purpose was to investigate how virtual laboratory simulations were integrated as part of the assessments of a practical-embedded…
Descriptors: Virtual Classrooms, Computer Simulation, COVID-19, Pandemics
