Among the efforts directed at remediating contaminated sites, the use of immobilized microorganisms is an appealing alternative because it offers several advantages over the use of free cells, including protecting cells from the toxic effects of hazardous compounds and increasing their survival and metabolic activity. "Pseudomonas…

Describes experiments suitable for introducing use of radioisotopes in biology. Includes demonstrations of tracing food chains, uptake of ions by plants, concentration of elements by insects, tracing photosynthetic reactions, activation analysis of copper, and somatic and genetic effects. Uses autoradiographic and counting techniques. (AL)

Describes a computer program that students use to check their suggestions of possible lines of investigation during a microbiology exercise, the results of procedures, and the decisions made on the basis of experimental evidence. Effectiveness of the program and practical aspects of the investigation are considered. (DH)

This paper reports on a simple hands-on laboratory procedure for high school students in studying both radiation damage and DNA repair systems in bacteria. The sensitivity to ultra-violet (UV) radiation of both "Escherichia coli" and "Serratia marcescens" is tested by radiating them for varying time periods. Two growth temperatures are used in…

Describes the hazards of using living materials in the science laboratory and outlines safety procedures that are particularly applicable to schools. Explains hazards to both mental and physical health and examines the latter in terms of fundamental and contingent factors. (Author/GS)

The food preservative sorbic acid can be extracted from Eurasian mountain ash berries (commercially available) and used to show antifungal properties in microbiological investigations. Techniques for extraction, purification, ultraviolet analysis, and experiments displaying antifungal activity are described. A systematic search for similar…

Describes some practical exercises involving the glutamine synthetase enzyme of Beneckea Natriegens. (CS)

Recommendations are made for handling and growth of Beneckea natriegens, a rapidly growing, nonpathogenic marine bacterium which can be used with advantage to replace Escherichia coli in many undergraduate microbiology experiments. Examples of experiments and the potential value of this organism as a subject for student research projects are…

A procedure that allows students to view an entire bacterial growth curve during a two- to three-hour student laboratory period is described. Observations of the lag phase, logarithmic phase, maximum stationary phase, and phase of decline are possible. A nonpathogenic, marine bacterium is used in the investigation. (KR)

Describes five microbiological experiments using the marine organism Vibrio natriegens. This organism is highly suitable for laboratory work because it is non-pathogenic and grows extremely rapidly, having the distinction of the lowest mean generation time yet recorded (9.8 minutes). (JR)

Describes simple quantitative determinations for ethanol and carbon dioxide, together with techniques for examining the effects of a number of environmental factors on their production. The experimental work centers around the growth of a cell population of yeast, and is suitable for senior high school students. (JR)

Reported is a technique for chromosome staining that is simple, rapid, inexpensive, and requires little skill for success. (PB)

Two implanted slide techniques are described by which activity of proteolylic bacteria and the growth of algae in the soil can be readily studied by school students using simple apparatus and methods. Variations are suggested for studying the effects of agricultural practices and environmental conditions on the soil bacteria and algae. (Author/DS)