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Direct linkThaveesangsakulthai, Isaya; Kulsing, Chadin – Journal of Chemical Education, 2021
Optimization of experimental conditions in chromatography is recognized as a wear-and-tear process involving hundreds of experiments in order to maximize the number of separated compounds for each sample. The critical thinking skills and practices entailed cannot be achieved with students in a conventional lecture class. This report provides a…
Descriptors: Spreadsheets, Simulation, Biochemistry, Laboratory Procedures
Marsiglia, William M.; Qamra, Rohini; Jackson, Kimberly M.; Traaseth, Nathaniel J. – Journal of Chemical Education, 2020
The design of undergraduate laboratory courses that provide meaningful research-based experiences enhances undergraduate curricula and prepares future graduate students for research careers. In this article, a course-based undergraduate research experience (CURE) laboratory module was designed for upper-division undergraduate biochemistry and…
Descriptors: Biochemistry, Science Instruction, College Science, Student Research
Silverstein, Todd P.; Kirk, Sarah R.; Meyer, Scott C.; Holman, Karen L. McFarlane – Biochemistry and Molecular Biology Education, 2015
We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure,…
Descriptors: Science Instruction, Science Laboratories, Science Process Skills, Spectroscopy
Ackerman, Megan M.; Ricciardi, Christopher; Weiss, David; Chant, Alan; Kraemer-Chant, Christina M. – Journal of Chemical Education, 2016
An undergraduate biochemistry laboratory experiment is described that utilizes free online bioinformatics tools along with readily available exonucleases to study the effects of base stacking and hydrogen bonding on the UV absorbance of DNA samples. UV absorbance of double-stranded DNA at the ?[subscript max] is decreased when the DNA bases are…
Descriptors: Undergraduate Students, Biochemistry, Laboratory Experiments, Science Experiments
Melanson, Michelle; Sood, Abha; Torok, Fanni; Torok, Marianna – Biochemistry and Molecular Biology Education, 2013
An undergraduate laboratory exercise is described to demonstrate the biochemical applications of electron paramagnetic resonance (EPR) spectroscopy. The beta93 cysteine residue of hemoglobin is labeled by the covalent binding of 3-maleimido-proxyl (5-MSL) and 2,2,5,5-tetramethyl-1-oxyl-3-methyl methanethiosulfonate (MTSL), respectively. The excess…
Descriptors: College Science, Science Laboratories, Undergraduate Study, Science Instruction
Wilson, Karl A.; Tan-Wilson, Anna – Biochemistry and Molecular Biology Education, 2013
Mass spectrometry (MS) has become an important tool in studying biological systems. One application is the identification of proteins and peptides by the matching of peptide and peptide fragment masses to the sequences of proteins in protein sequence databases. Often prior protein separation of complex protein mixtures by 2D-PAGE is needed,…
Descriptors: Spectroscopy, Biochemistry, Science Instruction, Molecular Biology
Arnold, Randy J.; Arndt, Brett; Blaser, Emilia; Blosser, Chris; Caulton, Dana; Chung, Won Sog; Fiorenza, Garrett; Heath, Wyatt; Jacobs, Alex; Kahng, Eunice; Koh, Eun; Le, Thao; Mandla, Kyle; McCory, Chelsey; Newman, Laura; Pithadia, Amit; Reckelhoff, Anna; Rheinhardt, Joseph; Skljarevski, Sonja; Stuart, Jordyn; Taylor, Cassie; Thomas, Scott; Tse, Kyle; Wall, Rachel; Warkentien, Chad – Journal of Chemical Education, 2011
A multivitamin tablet and liquid are analyzed for the elements calcium, magnesium, iron, zinc, copper, and manganese using atomic absorption spectrometry. Linear calibration and standard addition are used for all elements except calcium, allowing for an estimate of the matrix effects encountered for this complex sample. Sample preparation using…
Descriptors: Chemistry, Spectroscopy, Biochemistry, Nutrition
Shaver, Lee Alan; Leung, Sam H.; Puderbaugh, Amy; Angel, Stephen A. – Journal of Chemical Education, 2011
The determination of total phenolics in foods and fruit juices was used successfully as a laboratory experiment in our undergraduate general, organic, and biological (GOB) chemistry course. Two different colorimetric methods were used over three years and comparative student results indicate that a ferrous ammonium sulfate (FAS) indicator…
Descriptors: Chemistry, Laboratory Experiments, Food, Biochemistry
Mehl, Andrew F.; Crawford, Mary A.; Zhang, Lei – Journal of Chemical Education, 2009
Few laboratory procedures describe the use of circular dichroism (CD) at the undergraduate level. To increase the number of laboratory exercises using CD, a thermal denaturation study of myoglobin using CD is described to assess protein stability. Values obtained from a more classic linear data analysis approach are consistent with data analyzed…
Descriptors: Laboratory Procedures, Data Analysis, Science Experiments, Spectroscopy
Peer reviewedRinehart, Kenneth L., Jr. – Science, 1982
Discusses reactions and characteristics of fast atom bombardment (FAB) mass spectroscopy in which samples are ionized in a condensed state by bombardment with xenon or argon atoms, yielding positive/negative secondary ions. Includes applications of FAB to structural problems and considers future developments using the technique. (Author/JN)
Descriptors: Biochemistry, Chemical Analysis, Chemistry, College Science
Peer reviewedHoskins, L. C. – Journal of Chemical Education, 1984
Discusses the theory of resonance Raman (RR) spectroscopy as it applies to beta-carotene and lycopene pigments (found in tomatoes and carrots, respectively). Also discusses an experiment which demonstrates the theoretical principles involved. The experiment has been tested over a three-year period and has received excellent acceptance by physical…
Descriptors: Biochemistry, Chemical Analysis, College Science, Higher Education
Peer reviewedThierry-Palmer, M. – Journal of Chemical Education, 1984
Provides procedures for synthesizing and purifying a vitamin K metabolite (2,3-epoxide) to introduce many of the techniques used in lipid biochemistry. Includes typical results obtained as well as an optional experiment designed to test the purity of the epoxide obtained. (JM)
Descriptors: Biochemistry, Chemical Reactions, College Science, Higher Education
Peer reviewedO'Kennedy, Richard – Biochemical Education, 1988
Describes an instructional experiment that was designed to illustrate the use of periodate oxidation in sugar analysis, and the consequence that the absorbance of mixtures of non-interacting substances is additive. (TW)
Descriptors: Biochemistry, College Science, Higher Education, Laboratory Procedures
Peer reviewedLarsen, Peder Olesen; And Others – Journal of Chemical Education, 1982
Background information, laboratory procedures, and discussion of results are provided for experiments designed to familiarize students with the biochemistry of nicotinamide coenzymes and the chemistry involved in their reactions. In addition, students are exposed to ultraviolet (UV)-spectroscopy as a tool for investigating reaction mechanisms in…
Descriptors: Biochemistry, Chemical Reactions, College Science, Higher Education
Peer reviewedNitta, Yasunori; And Others – Journal of Chemical Education, 1984
Describes a set of experiments (for senior-level biochemistry students) which permit evaluation and estimation of rate and equilibrium constants involving an intermediate in the alpha-chymotrypsin mediated hydrolysis of ortho-hydroxy-alpha-toluenesulfonic acid (I). The only equipment required for the experiments is a well-thermostated double beam…
Descriptors: Biochemistry, Chemical Equilibrium, Chemical Reactions, College Science
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