Real-time quantitative PCR is a technique used to monitor the PCR reaction in real time. RT-qPCR is broadly classified into two types based on its purpose: absolute and relative quantification. Absolute quantification is used in a wide array of fields such as microbiology, food technology, and biotechnology to quantify the microbiological…

The human nose is a very sensitive detector and is able to detect potent aroma compounds down to low ng/L levels. These levels are often below detection limits of analytical instrumentation. The following laboratory exercise is designed to compare instrumental and human methods for the detection of volatile odor active compounds. Reference…

As advances in biotechnology and molecular biology rapidly expand in research settings, it is vital that we continue to prepare high school students to enter and thrive in those modern laboratories. This multistep, inquiry-based lab describes highly adaptable methods to teach students not only current molecular techniques and technologies, but…

Few laboratory procedures describe the use of circular dichroism (CD) at the undergraduate level. To increase the number of laboratory exercises using CD, a thermal denaturation study of myoglobin using CD is described to assess protein stability. Values obtained from a more classic linear data analysis approach are consistent with data analyzed…

Expression proteomics has become, in recent years, a key genome-wide expression approach in fundamental and applied life sciences. This postgenomic technology aims the quantitative analysis of all the proteins or protein forms (the so-called proteome) of a given organism in a given environmental and genetic context. It is a challenge to provide…

Describes an experiment which uses the Gran plot for analyzing free ions as well as those involved in an equilibrium. Discusses the benefits of using Gran plots in the study of acids, as well as other analytes in solutions. Presents background theory along with a description of the experimental procedures. (TW)

Discusses an experiment designed to introduce students to the basic principles of the fast Fourier transform and Fourier smoothing through transformation of time-dependent optical absorption data from an oscillating reaction. Uses the Belousov-Zhabotinskii reaction. Describes the experimental setup and data analysis techniques.

Describes an experiment for plotting equipotential curves in which the normally used electrometer is replaced by a null-deflecting potentiometer, and in which the uniform resistance wire is substituted by carbon-impregnated conductive paper. Procedures and sample plot/results (including graphic analysis) are provided. (JM)

Described is an experiment in which the student can ascertain the meaning of a negative result from a qualitative test by performing a more sensitive quantitative test on the same sample. Methodology for testing urinary glucose with a spectrophotometer at 630 nm and with commercial assaying glucose strips is presented. (MVL)

Describes an interactive computer program written to provide accurate and immediate feedback to students while they are processing experimental data. Discusses the problems inherent in laboratory courses that led to the development of this program. Advantages of the software include allowing students to work at their own pace in a nonthreatening…