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Alison Bates; Kathryn M. Williams; Ann E. Hagerman – Biochemistry and Molecular Biology Education, 2025
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…
Descriptors: Undergraduate Students, Science Instruction, Laboratory Experiments, Kinetics
Muhammad Saefi; Widi Cahya Adi; Amining Rahmasiwi; Hidayati Maghfiroh; M. Eval Setiawan; Miza Nina Adlini; Syarif Rizalia – Journal of Biological Education Indonesia (Jurnal Pendidikan Biologi Indonesia), 2023
Laboratory course activities often focus on "cookbook style," so their activities have not yet integrated with scientific thinking as an essential aspect. In this research, the laboratory experience of microbial analysis techniques was redesigned using alternative inquiry to involve students in isolating and characterising yeast to make…
Descriptors: Laboratory Experiments, Teaching Methods, Food, Cooking Instruction
Odunuga, Odutayo O.; Cheatwood, Nicholas Y.; Mullins, John A.; Nguyen, Samantha K.; Fry, Darrell R.; Harris, Michele R. – Journal of Chemical Education, 2021
This paper describes an undergraduate biochemistry laboratory module consisting of a set of experiments designed around a purification scheme for bovine serum albumin (BSA). Students purify BSA from cow plasma by a combination of salt and acetone precipitation, equilibrium dialysis, ion exchange, and size exclusion chromatography. Students use the…
Descriptors: Undergraduate Students, College Science, Science Instruction, Instructional Design
Marsiglia, William M.; Qamra, Rohini; Jackson, Kimberly M.; Traaseth, Nathaniel J. – Journal of Chemical Education, 2020
The design of undergraduate laboratory courses that provide meaningful research-based experiences enhances undergraduate curricula and prepares future graduate students for research careers. In this article, a course-based undergraduate research experience (CURE) laboratory module was designed for upper-division undergraduate biochemistry and…
Descriptors: Biochemistry, Science Instruction, College Science, Student Research
Marchioretto, Mira K.; Horton, Jacqueline T.; Berstler, Calvin A.; Humphries, Joseph B.; Koloditch, Isaac J.; Voss, Samuel D.; de La Harpe, Kimberly; Hicks, Barry W.; Jefferies, Latisha R. – Biochemistry and Molecular Biology Education, 2018
Förster resonance energy transfer (FRET) is the basis for many techniques used in biomedical research. Due to its wide use in molecular sensing, FRET is commonly introduced in many biology, chemistry, and physics courses. While FRET is of great importance in the biophysical sciences, the complexity and difficulty of constructing FRET experiments…
Descriptors: Undergraduate Students, Biomedicine, Laboratory Procedures, Laboratory Experiments
Jie Xu; Zhihua Shao; Song Jia; Jihong Sha; Jiao Li; Furong Gao; Xiujuan Shi; Juan Wang; Caixia Jin; Mei Jiang; Haibin Tian; Jinfeng Cao; Hu Pu; Lei Xu; Lixia Lu – Biochemistry and Molecular Biology Education, 2024
The laboratory practice "Primary culture and directional differentiation of rat bone marrow mesenchymal stem cells (BMSCs)" is part of a required course for sophomore medical students at Tongji university, which has been conducted since 2012. Blended learning has been widely applied in medical courses. Based on a student-centered…
Descriptors: Cytology, Biochemistry, Science Instruction, Laboratory Experiments
Maurye, Praveen; Basu, Arpita; Sen, Sohini; Biswas, Jayanta Kumar; Bandyopadhyay, Tapas Kumar; Naskar, Malay – Biochemistry and Molecular Biology Education, 2018
Recent advancements in biochemical sciences have helped the researchers to explore the molecular logic of life inclusive of its multifarious expressions and explain many facts about the structure and functions of cellular macromolecules. Due to its simple and cost-effective nature, polyacrylamide gel electrophoresis (PAGE) has become the most…
Descriptors: High School Students, Biochemistry, Laboratory Procedures, Science Instruction
Large, Delaney N.; Van Doorn, Nathaniel A.; Timmons, Shannon C. – Biochemistry and Molecular Biology Education, 2023
Laboratory courses should cultivate enthusiasm for research and an appreciation for real-world scientific challenges to retain undergraduate students and encourage them to pursue STEM-related careers. Course-based undergraduate research experiences (CURE) have emerged as an inclusive pedagogical model that facilitates laboratory skill development,…
Descriptors: Cancer, Laboratory Experiments, Undergraduate Students, STEM Careers
Weers, Paul M. M.; Prenner, Elmar J.; Curic, Spomenka; Lohmeier-Vogel, Elke M. – Biochemistry and Molecular Biology Education, 2016
The laboratory protocol presented here takes about 3 hours to perform and investigates protein and lipid interactions. Students first purify His6-tagged human apolipoprotein A-I (apoA-I) with Ni-NTA affinity resin in a simple batch protocol and prepare multilamellar vesicles (MLV) from pre-dried phospholipid films. When apoA-I is added to the MLV,…
Descriptors: Laboratory Experiments, Biochemistry, Science Instruction, Laboratory Procedures
Lefurgy, Scott T.; Mundorff, Emily C. – Biochemistry and Molecular Biology Education, 2017
Here, we present a 13-week research-based biochemistry laboratory curriculum designed to provide the students with the experience of engaging in original research while introducing foundational biochemistry laboratory techniques. The laboratory experience has been developed around the directed evolution of an enzyme chosen by the instructor, with…
Descriptors: Biochemistry, Science Instruction, Laboratory Experiments, Curriculum Development
Applegate, Kaitlin B.; Cheek, Patrick R.; Inlow, Jennifer K. – Biochemistry and Molecular Biology Education, 2019
Laboratory exercises for undergraduate biochemistry students are described in which changes in sugar content during fermentation of the trendy beverage kombucha are analyzed by three methods: thin layer chromatography, a 3,5-dinitrosalicylic acid assay, and a standard commercial blood glucose meter. Each of the three analyses can be completed in a…
Descriptors: Majors (Students), Chemistry, Biology, College Science
Willbur, Jaime F.; Vail, Justin D.; Mitchell, Lindsey N.; Jakeman, David L.; Timmons, Shannon C. – Biochemistry and Molecular Biology Education, 2016
The development and implementation of research-inspired, discovery-based experiences into science laboratory curricula is a proven strategy for increasing student engagement and ownership of experiments. In the novel laboratory module described herein, students learn to express, purify, and characterize a carbohydrate-active enzyme using modern…
Descriptors: Biochemistry, Laboratory Experiments, Science Experiments, College Science
Silverstein, Todd P.; Kirk, Sarah R.; Meyer, Scott C.; Holman, Karen L. McFarlane – Biochemistry and Molecular Biology Education, 2015
We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure,…
Descriptors: Science Instruction, Science Laboratories, Science Process Skills, Spectroscopy
Ackerman, Megan M.; Ricciardi, Christopher; Weiss, David; Chant, Alan; Kraemer-Chant, Christina M. – Journal of Chemical Education, 2016
An undergraduate biochemistry laboratory experiment is described that utilizes free online bioinformatics tools along with readily available exonucleases to study the effects of base stacking and hydrogen bonding on the UV absorbance of DNA samples. UV absorbance of double-stranded DNA at the ?[subscript max] is decreased when the DNA bases are…
Descriptors: Undergraduate Students, Biochemistry, Laboratory Experiments, Science Experiments
Brunauer, Linda S.; Caslavka, Katelyn E.; Van Groningen, Karinne – Journal of Chemical Education, 2014
A multiday laboratory exercise is described that is suitable for first-year undergraduate chemistry, biochemistry, or biotechnology students. Students gain experience in performing chromatographic separations of biomolecules, in both a column and thin layer chromatography (TLC) format. Students chromatographically separate amino acids (AA) in an…
Descriptors: Science Experiments, Laboratory Experiments, College Science, Undergraduate Students