By utilizing targeted professional learning and predifferentiated, tiered, and enriched mathematics curriculum, teachers gained an understanding of differentiation of content and the value of differentiating content for their students. They also reported a stronger understanding of how to differentiate content in the classroom. However, several barriers to differentiation were also reported, which may provide educational researchers and professional learning coordinators further information about what hinders teachers' application of differentiated strategies, despite increased understanding and existing positive perceptions.