This study describes publication and career outcomes associated with junior faculty receiving the FOCUS Junior Faculty Investigator (JFI) Grant Award in Women's Health and Gender-based Medicine. Our data suggest that the JFI Grant Award helped foster the careers of research-oriented junior faculty as measured by publications related to the grant, current position, amount of time devoted to research, and self-reported impact. Investments in junior faculty research grants are associated with down-stream faculty career outcomes and research publications that appear important in supporting the advancement of biomedical researchers and clinician-scientists and may influence interest in women's health and gender-based medicine.