Behind the creativities and innovations in human history, there lie the humble practices of multidisciplinary studies that drive new revolutionary ideas and unconventional approaches. One such example is Leonardo da Vinci's "Mona Lisa"--one of the perennial portraits that bear witness to a transcendent level of creative art. It is one of his unique touchstones where he played with light and shade in such a way that both motion and emotion are entwined to the highest point of perfection in the ecstatic smile of the "Mona Lisa." By explaining how Leonardo created an unfathomable emotion in her smile and veiled her face by optics of uncertainty through the fusion of art and science, here we show why and how it can be considered as a prototype for multidisciplinary science education. The analysis of the inner details together with Leonardo's scientific epistemology advocates the importance of putting into practice repeated experiments and observations on widely diverse faculties for the emergence of innovative ideas, for the depiction of reality and for fostering creations.