To practice physical therapy, physical therapist assistants (PTAs) must graduate from an accredited program and pass the National Physical Therapy Examination (NPTE). There is a lack of evidence exploring the relationships between admission processes in PTA programs with no pre-requisites. The first purpose of this study was to determine whether differences exist in the first-time pass rate on the NPTE between students in a PTA program who completed Anatomy and Physiology I versus students who have not completed it before admission. The second purpose was to determine whether differences exist in first-time pass rate on the NPTE between students in a PTA program who completed College Algebra versus students who have not completed it before admission. Data from five graduating cohorts who were enrolled in a PTA program from 2016 through 2021 (N = 110) were retrospectively reviewed utilizing an alpha level of p < 0.05 to establish statistical significance. The study's results revealed that Anatomy and Physiology I was an indicator of NPTE first-time success with a statistically significant difference (p = 0.005) in the first-time pass rate success of students taking it prior to admission compared to those who took it after admission. College Algebra was an indicator of NPTE first-time success with a statistically significant difference (p = 0.002) in the first-time pass rate success of students taking it before admission compared to those who took it after admission. The study's results suggest that PTA programs requiring these two pre-requisites may demonstrate better first-time NPTE pass rates. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com/en-US/products/dissertations/individuals.shtml.]