In this study, we develop an interactive AR Learning System based on Augmented Reality and interactive touch-screen. The learning content knowledge is about conservation of fish in Taiwan. The system combines the game by the concept of AR book which allows children to learn about the importance of conservation of fish. A mechanism is designed to verify whether the game based on AR book well delivers the educational messages. The whole mechanism is an interactive game using touch screen to interact with the rod as a tool to get rid of the shackles of keyboard and mouse. It provides a more realistic interaction. The evaluation results show that the interactive AR Learning System has positive usability. (Contains 12 figures and 3 tables.)