Given that conservation education is a primary goal for zoos, it is important to study how changes in exhibit design in the same facility (with the same animals and visitor base) can impact short- and long-term conservation knowledge, attitudes, and behavior (KAB) amongst visitors. However, there is very limited research on this topic. Our goal was to conduct a comprehensive post-occupancy evaluation to study this impact at two exhibits that underwent renovations at Zoo Atlanta, using rigorous methodology and addressing limitations in previous research. We did not find significant differences in KAB scores when comparing the pre- and post-renovated exhibits. This is contrary to the existing view that renovations from non-naturalistic to naturalistic exhibits (and/or renovations to displays and signage) will improve KAB. Future researchers should continue to use rigorous methods to obtain valid and reliable measures of the impact of zoo exhibits and exhibit renovations on conservation-related KAB.