The present study evaluates high school juniors and seniors (n = 295) to explore their preference for science as indicated by science motivation, attitude, academic experience, and interest. Latent profile analysis was used to model profiles of preferences for science with a person-centered approach. Then, the impact of self-concept variables was explored and four profiles of science interest were identified. Gender differences were of particular interest due to concerns noted in the literature, and some gender differences were identified in the present study. Covariate analysis indicated vocabulary ability and personality as significantly different for students in the high science interest profile. Implications of these results and future research directions are discussed.