A laboratory experiment has been devised to train new graduate trainees in the safe and controlled handling of air-sensitive organometallic reagents using Schlenk lines, high vacuum pumps, liquid nitrogen traps, and cannula transfers. The reaction involves the formation of air-, moisture-, and thermally sensitive 2-pyridyllithium, generated in…

A Microsoft Excel workbook has been developed to simplify the quantitative analysis of experimentally measured titration curves for diprotic and triprotic amino acids such as glycine, arginine, histidine, and glutamate. Students perform the titration, enter the data into the worksheet, and manually adjust the resulting pK[subscript a] values to…

KinSim is a research-grade, interactive, user-friendly, open-source, and visual software for kinetics modeling of environmental chemistry and other applications. Students without any computer-programming background and limited knowledge of environmental chemistry can use KinSim, which also includes multiple features and functionality dedicated to…

This paper summarizes a project seminar in analytical chemistry that includes a method comparison approach and collaborative learning. In this project seminar, 12 students worked in four groups, each of which focused on one method for quantitative element analysis, including flame atomic absorption spectroscopy, inductively coupled plasma mass…

We demonstrate here a novel laboratory experiment for the synthesis of gold nanoparticles (AuNPs) by using a low energy gold-sputtering method together with a modern, green, and biofriendly deep eutectic solvent (DES). The strategy is straightforward, economical, ecofriendly, rapid, and clean. It yields uniform AuNPs of 5 nm in diameter with high…

The elementary reaction sequence A ? I ? Products is the simplest mechanism for which the steady-state and quasi-equilibrium kinetic approximations can be applied. The exact integrated solutions for this chemical system allow inferring the conditions that must fulfill the rate constants for the different approximations to hold. A graphical…

Galen of Pergamon (130-200 A.D.) was an accomplished showman and scientist who made enormous advancements in the understanding of the heart, nervous system, and mechanics of breathing. These advancements were often achieved during impressive public "performances" of vivisection on Barbary apes and other living animals. These "shock…

Circular dichroism (CD) has emerged as one of the standard biophysical techniques for the study of guaninequadruplex (G4) folding, cation effect, and ligand binding. The utility of this technique is based on its robustness, ease of use, and requirement of only small quantities of nucleic acid. This experiment is also extendable to the classroom…

"In situ" hybridization is a widely used technique for studying gene expression. Here, we describe two experiments addressed to postgraduate genetics students in which the effect of transcription factors on gene expression is analyzed in "Drosophila embryos of different genotypes by whole-mount in situ hybridization. In one of the…

The increased worldwide exploitation of nanomaterials has reinforced the importance of introducing nanoscale aspects into the undergraduate and graduate curriculum. To meet this need, a novel nano-laboratory module was developed and successfully performed by science and engineering students. The main goal of the experiment was to accurately…

Reaction workup can be a complex problem for those facing novel synthesis of difficult compounds for the first time. Workup problem solving by systematic thinking should be inculcated as mid-graduate-level is reached. A structured approach is proposed, building decision tree flowcharts to analyze challenges, and an exemplar flowchart is presented…

In this computational experiment, students use advanced quantum chemistry tools to simulate the photochromic reaction mechanism in naphthopyran derivatives. The first part aims to make students familiar with excited-state reaction mechanisms and addresses the photoisomerization of the benzopyran molecule by means of semiempirical quantum chemical…

This adaptable graduate laboratory course on protein purification offers students the opportunity to explore a wide range of techniques while allowing the instructor the freedom to incorporate their own personal research interests. The course design involves two sequential purification schemes performed in a single semester. The first part…

Remote access to real experiments is crucial for distance learners to experience the sciences. The exploitation of technology for this purpose is advantageous in global teaching and in exchange of ideas on a single front irrespective of distance barriers. Implementation of the distance method leads to cost-effective integrated-e-learning where…

Superposition gives rise to the probabilistic nature of quantum mechanics and is therefore one of the concepts at the heart of quantum mechanics. Although we have found that many students can successfully use the idea of superposition to calculate the probabilities of different measurement outcomes, they are often unable to identify the…