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Alison Bates; Kathryn M. Williams; Ann E. Hagerman – Biochemistry and Molecular Biology Education, 2025
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…
Descriptors: Undergraduate Students, Science Instruction, Laboratory Experiments, Kinetics
Naoto Shirasu; Shin'ichiro Yasunaga – Biochemistry and Molecular Biology Education, 2024
Here, we propose a laboratory exercise to quickly determine single nucleotide polymorphisms (SNPs) in human "alcohol dehydrogenase 1B (ADH1B)" and "aldehyde dehydrogenase 2 (ALDH2)" genes involved in alcohol metabolism. In this exercise, two different genotyping methods based on polymerase chain reaction (PCR), namely…
Descriptors: Genetics, Biochemistry, Students, Science Instruction
Katharine Hubbard; Marlena Birycka; Maisie-Elizabeth Britton; Joseph Coates; Isla Delphine Coxon; Chloe Hannah Jackson; Casper Leigh Nicholas; Tyler M. Priestley; J. J. Robins; Paula R. Ryczko; Talia Salisbury; Megan Shand; George Snodin; Beth Worsley – Journal of Biological Education, 2024
Providing hands-on practical education without access to laboratories during the COVID-19 pandemic has required creativity and innovation. In this paper, co-authored by academic staff and students, we describe an at-home mobile phone-based 'spectrophotometer' experiment used in an introductory undergraduate biology course. Using colour picker…
Descriptors: Hands on Science, Science Laboratories, Distance Education, Telecommunications
Ce´sar Pastrana-Pineda; Luis J. Melgoza-Rami´rez; Erandi M. Islas-Flores; Wendy L. Go´mez-Monsiva´is; Jero´nimo Gonce; Noe´ Lo´pez; Jose´ B. Limo´n-Gonza´lez; Jose´ S. Rodri´guez-Zavala; Emma Saavedra; Javier A. Belmont-Di´az – Journal of Chemical Education, 2025
The study of energy metabolism is fundamental in multiple disciplines, such as biochemistry, bioengineering, biomedicine, etc. Traditionally, in high school and college level courses, students are taught energy metabolism (glycolysis, Krebs cycle, and oxidative phosphorylation) through diagrams of metabolic pathways that depict the transformation…
Descriptors: Science Instruction, Science Laboratories, Biochemistry, Energy
Mark A. Chrisman; Michael J. Goldcamp; Alexis N. Rhodes; Jared Riffle – Journal of Chemical Education, 2023
This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…
Descriptors: Science Laboratories, Science Experiments, Laboratory Experiments, Science Instruction
Molecular Structure from Models to Mastery: An Inquiry-Based Study of Human Insulin-Degrading Enzyme
Michael A. Araujo; Alexandra A. Barrere; Selena-Rae Tirado; Candace E. Williams; Monica I. Strada; Benjamin J. Alper – Journal of Chemical Education, 2022
Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed…
Descriptors: Molecular Structure, Science Instruction, Science Laboratories, Science Experiments
Katherine R. McCance; Ana Maria Topliceanu; Darlene Echeverria; Shana L. McAlexander; Margaret R. Blanchard; Richard A. Venditti – Journal of Chemical Education, 2023
As e-commerce increases, the demand for packaging materials and the potential for generating waste and negative environmental impacts also rise. Packing peanuts are a type of plastic packaging material that are used to protect goods during the shipping process. Petroleum-based plastics are common packaging materials due to their low cost, light…
Descriptors: Chemistry, Plastics, Recycling, Conservation (Environment)
Muhammad Saefi; Widi Cahya Adi; Amining Rahmasiwi; Hidayati Maghfiroh; M. Eval Setiawan; Miza Nina Adlini; Syarif Rizalia – Journal of Biological Education Indonesia (Jurnal Pendidikan Biologi Indonesia), 2023
Laboratory course activities often focus on "cookbook style," so their activities have not yet integrated with scientific thinking as an essential aspect. In this research, the laboratory experience of microbial analysis techniques was redesigned using alternative inquiry to involve students in isolating and characterising yeast to make…
Descriptors: Laboratory Experiments, Teaching Methods, Food, Cooking Instruction
Kater?ina Trc?kova´; Hana Tkac?i´kova´; Roman Mars?a´lek – Journal of Chemical Education, 2023
The purpose of this article is to describe the possibilities of using worksheets in the teaching of lipids and proteins. The worksheet includes a concept map, suggestions for three safe experiments that can be performed with available household chemicals, and observation results. The worksheets were implemented into action research in two classes…
Descriptors: Laboratory Experiments, Laboratory Safety, Worksheets, Science Instruction
Tyler A. Shaffer; Carlos U. Herrada; Avery M. Walker; Laura D. Casto-Boggess; Lisa A. Holland; Timothy R. Johnson; Megan E. Jones; Yousef S. Elshamy – Journal of Chemical Education, 2023
Electrophoresis is integral to analytical and biochemistry experiences in undergraduate education; however, fundamental principles of the method are often taught in upper-level laboratories through hands-on experiences. A laboratory activity is reported that teaches the concepts of electrophoretic mobility and electroosmotic flow. A single…
Descriptors: Chemistry, Science Instruction, Teaching Methods, Cost Effectiveness
Anna Laguta; Natalya Vodolazkaya; Dmitry Nerukh – Journal of Chemical Education, 2024
Chemistry experiments involving biological aspects are in high demand. This laboratory exercise blends biochemistry and physical chemistry, illustrates key concepts of the virus capsid surface, and is designed for a chemistry laboratory course for students majoring in biology in college. The experimental part provides a mastery of…
Descriptors: Undergraduate Students, Science Instruction, Chemistry, Biochemistry
Keithley, Richard B.; Sullivan, Dylan T.; Dodd, John M., II; Iyer, Kavya V.; Sarisky, Catherine A.; Johann, Timothy W. – Journal of Chemical Education, 2021
Techniques and instrumentation that involve fluorescence are used in many careers related to biology and biochemistry. However, due to the expense of instrumentation, the theory and practice of fluorescence spectroscopy is commonly concentrated in instrumental analysis in most curricula, a class rarely required for biology and biochemistry majors.…
Descriptors: Chemistry, Science Instruction, College Science, Undergraduate Study
Sedky, Nada K.; Arafa, Reem K. – Biochemistry and Molecular Biology Education, 2022
Nowadays, novel Biochemistry lab techniques are being introduced at a very fast pace in scientific research. This requires development of new labs for undergraduate Biochemistry courses to equip the students with up-to-date techniques. However, the time limit of Biochemistry labs for undergraduate students represents a major obstacle. This article…
Descriptors: Biochemistry, Science Instruction, Undergraduate Study, College Science
Ahlia Khan-Trottier – Biochemistry and Molecular Biology Education, 2024
The COVID-19 pandemic has forced a shift in thinking regarding the safe delivery of wet laboratory courses. While we were fortunate to have the capacity to continue delivering wet laboratory experiments with physical distancing and other measures in place, modifications to the mechanisms of delivery within courses were necessary to minimize risk…
Descriptors: Computer Software, Science Laboratories, Science Instruction, Student Journals
Stefan J. Hammerschmidt; Fabian Barthels; Annabelle C. Weldert; Christian Kersten – Journal of Chemical Education, 2024
Isothermal titration calorimetry (ITC) is the method of choice for thermodynamic binding profiling. As a label-free in-solution technique, ITC experiments do not require modified or immobilized proteins or ligands and hence can be performed under close-to-native environmental conditions. It directly follows the released heat of the molecular…
Descriptors: Graduate Students, Chemistry, Science Instruction, Graduate Medical Education