As a universal and extensively adopted technique, enzyme-linked immunosorbent assay (ELISA) can be used to detect and quantify small molecules in many applications both clinical and analytical. However, generally, students experiment mechanically using commercial ELISA kits according to the instructions and eventually produce a standard curve to…

Students in high school tend to find genetics difficult to learn because it requires some crosslinked knowledge about subjects like genes, proteins, cells, tissues as well as organs and systems. It also becomes more difficult to connect and explain concepts such as the gene expression at the level of the cell to conceptualize the effect of the…

CRISPR (also known as CRISPR-Cas9) is a powerful biotechnology tool that gives scientists unprecedented access to the genetic makeup of all living organisms, including humans. It originally evolved as an adaptive immune system in bacteria to defend against viruses. When artificially harnessed in the laboratory it allows scientists to accurately…

In theory, there is broad agreement among science education stakeholders that supporting the achievement of scientific literacy is crucial for citizens to make informed and critical decisions about socioscientific issues (SSI). Likewise, it is widely held that argumentation is an important component of scientific literacy. In practice, however,…

We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published…

This study explored the effects of directed and undirected online peer feedback types on students' peer feedback performance, argumentative essay writing skills, and acquisition of domain-specific knowledge. The study used a pre-test and post-test design with four conditions (feedback, feedforward, a combination of feedback and feedforward, and…

This study aimed at designing instructional material named Biotechnology Instruction Module (BIM) which is enriched with active learning methods to enhance the 8th grade students' learning of the topic. The development of the teaching materials proceeded within the stages of the ADDIE instructional design model. The final form of the instructional…

This laboratory experiment describes the production and purification of plasmid DNA for undergraduate biochemistry and biotechnology courses. This experiment performed in a one-week period includes the protocols for plasmid pVAX1-LacZ production in "Escherichia coli" DH5[alpha] cells and subsequent purification of supercoiled pVAX1-LacZ.…

The science curriculum in the Turkish education system includes socioscientific issues (SSI) as a context for developing students' reasoning skills, scientific thinking habits, and decision-making skills from an early age. Conducting activities on real problems with teachers and pre-service teachers based on SSI and making decisions considering…

Undergraduates learn that gene editing in diverse organisms is now possible. How targeted manipulation of genes and genomes is utilized in basic science and biomedicine to address biological questions is challenging for undergraduates to conceptualize. Thus, we developed a lab experience that would allow students to be actively engaged in the full…

Clustered regularly interspaced short palindromic repeats (CRISPR) are a revolutionary tool based on a bacterial acquired immune response system. CRISPR has gained widespread use for gene editing in a variety of organisms and is an increasingly valuable tool for basic genetic research, with far-reaching implications for medicine, agriculture, and…

Students will achieve the expected science education outcomes by giving more chances for scientific argumentation through building an argument, considering it, and debating various explanations on the phenomena. The learning activity is a complex and fast process, therefore the use of video can be a solution since the learning process can be…

With the advancement of ribonucleic acid (RNA) research in the field of biochemistry, ensuring that undergraduate students have both the theoretical and practical knowledge of current, cutting-edge experimental techniques is of marked importance. Many current biochemistry experiments demonstrate various basic molecular biological techniques…

Over the past few years, a new research field has emerged, focusing on the social-scientific criteria for the study of opinions toward genetically modified foods (GMFs), since these may be limiting factors for the success or failure of these products. Basic education is the first step in the Mexican education system, and teachers may wield an…

Teaching the Genome Generation (TtGG) is a teacher professional development program and set of high school biology lessons that support interwoven classroom instruction of molecular genetics, bioinformatics, and bioethics. Participating teachers from across New England implement the modular elements of program at a high rate in a variety of…