We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…

This article aims to simplify and facilitate the process of practical teaching of enzyme kinetics by utilizing minimal teaching laboratory requirements. Simultaneously, it ensures that students comprehend the enzyme kinetics experiment effectively. The focus is on teaching students how to estimate the maximum velocity (Vmax) and Michaelis constant…

Scientific reasoning competencies enable students to engage in scientific inquiry through a wide range of methods, for example by asking questions, formulating hypotheses, and planning, carrying out, and evaluating experiments. Laboratories are particularly suitable to foster these competencies in higher education. In order to give lab educators…

Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

The pharmaceutical and medicine manufacturing industry has become the largest industrial sector for the employment of chemists, indicating a need for experiments with a pharmaceutical sciences context in the undergraduate chemistry curriculum. In the pharmaceutical industry, testing drug dissolution is a key analytical task for solid oral dosage…

This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel­(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…

An integrated system comprising a commercial high-performance liquid chromatography HPLC (except autosampler) equipped with an external multichannel autofeeding module was developed to accomplish interference-free separation and kinetic tracking for the H[subscript 2]O[subscript 2]-thiourea reaction, which is proposed as a lab experiment for…

We are living in the Big Data era, and yet we may have serious troubles when dealing with a handful of kinetic data if we are not properly instructed. The aim of this paper, related to enzyme kinetics, is to illustrate how to determine the K[subscript m] and V[subscript max] of a michaelian enzyme avoiding the pitfalls in which we often fall. To…

This experimental lab set is for students taking college chemistry courses. It includes four laboratory works and completely illustrates the important parts of chemical kinetics, namely the determination of the rate constant and the determination of the factors influencing its value. They can be used in a laboratory set for a physical chemistry…

Millions of people do not have access to clean drinking water; thus, cost-efficient water treatment systems are vital. Chemists, environmentalists, technicians, and engineers will be the professionals making breakthroughs in this industry. This laboratory experiment aims to introduce undergraduate students to the removal of pollutants from water…

This practical is aimed at students who have been introduced to the tableting process, drug delivery systems, and dissolution. The student can design and manufacture a tablet investigating the effect of excipients and their role in tablet design and then generate a release profile of the tablet by using a dissolution apparatus, flow-through cell,…

Optimization is a component critical to method development. Mathematical modeling of physical phenomena enables an efficient search for the parameters and conditions that optimize methods and systems. Here, optimization of methods in sonoelectrochemistry, electrochemical separations, electrochemical education, and assessment of diabetic status are…

Recently, scholars have suggested a co-design collaboration with instructors and students to effectively implement formative assessment (FA) practices because it ensures a high-quality design that considers users' needs, values, and goals in a specific learning context. This study examines the effect of co-designed FA practices, in which…

In this guided inquiry experiment, students extract catecholase enzyme from apples to catalyze the oxidation of catechol. They follow the reaction using the UV--vis absorbance of the "p"-benzophenone produced to determine the Michaelis-Menten kinetic parameters. Students make selected experimental choices within a structured framework…

Oxygen reduction reaction (ORR),as a multielectron electrode reaction, is an important electrochemical reaction in the field of electrochemical energy storage systems. Herein, a new advanced electrochemical laboratory experiment is designed for undergraduates to master the preparation of oxygen reduction catalytic electrode. Students can use…