Tofu, a traditional Chinese food, is now popular worldwide. However, few people notice the chemistry that is involved in its production. To shed light on this, we have designed a simple demonstration for lower-level undergraduates in organic chemistry or biochemistry courses to help them understand the chemistry principles that underlie the…

The construction and characterization of a glucose biosensor has been implemented in an instrumental chemical analysis course to provide students experience with chemical sensing, electroanalytical methods, and flow injection analysis (FIA). The glucose biosensor was assembled on a glassy carbon electrode by depositing a layer of Prussian blue…

Enzyme-like catalysis is the use of artificial catalysts to catalyze chemical reactions, which has the characteristics of enzymatic catalysis, such as high selectivity, high efficiency, and mild reaction conditions. In recent years, 2D materials, thickness ranging from a single to several atomic layers, have become a hot research topic in the…

Enzymes, enzymatic kinetics, and enzyme inhibition are topics covered in undergraduate biochemistry textbooks, having perceptible relevance to everyday life. The experimental use of enzymes involved in basic physiologic processes may be of utmost pedagogic interest because they bring evidence on how a significant number of drugs are used to…

Traditional undergraduate biochemical laboratory curricula are confronted with challenges in improving student-learning outcomes while incorporating exciting and thought-provoking research-based laboratories due to limitations with time, increased student enrollment, and high-cost laboratory resources. However, with specialized biochemical…

Providing hands-on practical education without access to laboratories during the COVID-19 pandemic has required creativity and innovation. In this paper, co-authored by academic staff and students, we describe an at-home mobile phone-based 'spectrophotometer' experiment used in an introductory undergraduate biology course. Using colour picker…

Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed…

Structure--activity relationships are foundational concepts in biochemistry and drug design. Despite their importance, it can be difficult to create and execute laboratory activities that allow students to directly observe the interactions between small-molecule drugs and their biomolecular targets. Here, we report an activity that investigates…

This report describes a laboratory experiment for an undergraduate-level inorganic chemistry or biochemistry course involving the study of the kinetics of the catecholase activity of a synthetic nickel­(II)-oximate complex. A model substrate, 3,5-di-tert-butylcatechol(DBC), undergoes aerobic oxidation to 3,5-di-tert-butylbenzoquinone (DBQ) in the…

Here we describe an approach and overall concept of how to train undergraduate university students to understand basic regulation and integration of glucose and fatty acid metabolism in response to fasting, intake of carbohydrates, and aerobic exercise. During lectures and both theoretical and practical sessions, the students read, analyze, and…

While metabolism is a fundamental process in physiology, the measurement of metabolism by living animals often requires specialized and expensive equipment. In 2016, I developed a novel laboratory exercise for animal physiology students where students were able to observe the effects of temperature and long-term temperature acclimation on the…

Students encounter antioxidants in many areas of their daily life. Antioxidants play a crucial role in cosmetics, in nutritional or dietary supplements, as additives for the preservation of food, and as a natural component in fruit and vegetables. Accordingly, antioxidants are believed to promote health through the prevention of oxidative stress…

Pulse oximetry has become the standard of care in operating rooms, intensive care units, and hospitals worldwide. A pulse oximeter continuously and noninvasively monitors the functional oxygen saturation of hemoglobin in arterial blood (Sa[subscript O2]). Sa[subscript O2] is so important in medical care that it is often regarded as a fifth vital…

A process for generating and writing an individual research proposal with or without access to advanced experimental techniques is described herein. This process was first included in a glycoconjugate biochemistry course to increase student interaction with more areas of the field, but the process was designed to be applicable elsewhere as well.…

Guided inquiry is the practice of allowing students to come to their own conclusions about a set of data instead of verifying a known result using a given experimental procedure. Open inquiry takes this idea one step further by allowing students to develop their own experiment and then analyze the data to arrive at a conclusion. To expose upper…