Questioning is an important activity in teaching. In medical colleges, on-stage quiz competitions are appreciated by students as well as faculty as they are an engaging way to connect with the discipline. We organized the Physiology Quiz Competition to assess the concepts of functional mechanisms of various organ systems. It was an academic tool for teaching and learning for 200 first-year Bachelor of Medicine and Bachelor of Surgery (MBBS) course students. It was conducted in four rounds: multiple choice question-based round 1 (R1), explanatory-type round 2 (R2), rapid-fire round 3 (R3), and image-based round 4 (R4). The postquiz feedback questionnaire included 23 total questions; 10 questions used a 5-point Likert scale, another 10 questions had dichotomous options, and the remaining 3 questions were used to collect general information about all rounds. Data were collected and analyzed. The outcome of academic learning was reported by 26% of students regarding R1, 30.5% regarding R2, 18.5% regarding R3, and 25% regarding R4 round. R4 and R3 were reported by 44.5% and 23% of students and R2 and R1 by 16% of students as a source of entertainment. A total of 67% of students found the quiz to be an excellent teaching and learning method. All students endorsed the quiz as innovative and interesting. In conclusion, the Physiology Quiz Competition can be used for edutainment as an innovative teaching and learning method, especially for first-year medical students.