A laboratory experiment was designed to introduce the practical application of biocatalysis in Bachelor and Master courses in biotechnology and medicinal chemistry, respectively. To this aim, the lipase from "Candida rugosa" was characterized (protein concentration and activity), immobilized on a carrier (by hydrophobic adsorption), and…

Enzymes are widely applied in different industries, like pharmaceutical, food, biofuel, and waste valorization. However, the enzyme operating conditions are very limited. So, in most industrial processes, enzymes are not efficient, due to the use of harsh conditions, like organic solvents, extreme pH, and temperature. Immobilization arises as a…

Human ageing and age-associated diseases are among the greatest challenges and financial burdens faced by developed countries. Unfortunately, undergraduate students often do not experience any laboratory training in ageing research in the current laboratory curriculum of majors in biology, biotechnology, or medicine. In this study, we developed…

The aim of this study is to determine the trend of studies in the laboratory and put the current situation in Turkey. For this purpose, document analysis technique, one of the qualitative research methods, was used in the research. The data group of the research consists of thesis studies on laboratories in our country between 1999-2017. Theses in…

Clustered regularly interspaced short palindromic repeats (CRISPR) are a revolutionary tool based on a bacterial acquired immune response system. CRISPR has gained widespread use for gene editing in a variety of organisms and is an increasingly valuable tool for basic genetic research, with far-reaching implications for medicine, agriculture, and…

Homologous recombination, a central concept in biology, is defined as the exchange of DNA strands between two similar or identical nucleotide sequences. Unfortunately, undergraduate students majoring in biotechnology often experience difficulties in understanding the molecular basis of homologous recombination. In this study, we developed and…

This article describes the assembly of a simple, low-cost, high-performance liquid chromatography (HPLC) system and its use in the undergraduate chemical engineering laboratory course to perform simple experiments. By interpreting the results from these experiments students are able to gain significant experience in the general method of…

We present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a…

Messenger ribonucleic acid (mRNA) plays a pivotal role in the central dogma of molecular biology. Importantly, molecular events occurring during and after mRNA synthesis have the potential to create multiple proteins from one gene, leading to some of the remarkable protein diversity that genomes hold. The North Carolina State University…

The increasingly interdisciplinary nature of today's scientific research is leading to the transformation of undergraduate education. In addressing these needs, the University of Houston's College of Technology has developed a new interdisciplinary research-based biotechnology laboratory curriculum. Using the pesticide degrading bacterium,…

This study explored the effectiveness of an inquiry-based cellulase laboratory unit in promoting inquiry in undergraduate students in biotechnology. The following tools were used to assess the students' achievements and attitude: conceptual understanding test, concept mapping, students' documents, CLES questionnaire, students' self reflection, and…

The health industry is an important and growing economic engine. Advances are being made in pharmaceutical and biotechnology discoveries and their applications (including manufacturing), as well as in health care services. As a result, there is an increasing sophistication of the products and services available and being developed, with an…

The purpose of this article is to discuss "small-group apprenticeships (SGAs)" as a method to instruct cell culture techniques to high school participants. The study aimed to teach cell culture practices and to introduce advanced imaging techniques to solve various biomedical engineering problems. Participants designed and completed experiments…