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Li, Chanjuan; Wu, Gaobing; Fan, Xuezhu; Guo, Siqi; Qin, Yuqing; Hu, Yonggang; Ruan, Lifang – Biochemistry and Molecular Biology Education, 2023
This article describes a comprehensive practical laboratory method for developing an enzyme to more easily measure glyphosate levels in solution. Through this article, undergraduate students of biology majors can conduct research experiments in critical fields by utilizing various techniques, such as chemiluminescence (CL) biosensors with…
Descriptors: Biochemistry, Scientific Concepts, Biotechnology, Laboratory Experiments
Marina S. Robescu; Daniela Ubiali; Riccardo Semproli; Teodora Bavaro – Journal of Chemical Education, 2024
A laboratory experiment was designed to introduce the practical application of biocatalysis in Bachelor and Master courses in biotechnology and medicinal chemistry, respectively. To this aim, the lipase from "Candida rugosa" was characterized (protein concentration and activity), immobilized on a carrier (by hydrophobic adsorption), and…
Descriptors: Science Laboratories, Laboratory Experiments, Science Experiments, College Science
Gomes, Diana; Sousa, Angela; Passarinha, Lui´s A. – Journal of Chemical Education, 2021
Enzymes are widely applied in different industries, like pharmaceutical, food, biofuel, and waste valorization. However, the enzyme operating conditions are very limited. So, in most industrial processes, enzymes are not efficient, due to the use of harsh conditions, like organic solvents, extreme pH, and temperature. Immobilization arises as a…
Descriptors: Science Instruction, Science Laboratories, Laboratory Experiments, Science Experiments
del Rosario Cappellari, Lorena; Chiappero, Julieta; Banchio, Erika – Biochemistry and Molecular Biology Education, 2019
Rhizobacteria that colonize plant roots and promote plant growth are referred to as plant growth-promoting rhizobacteria, and this can stimulate plant growth either indirectly or directly. Volatile organic compounds (VOCs) emitted by rhizobacteria have the capacity to promote plant growth as well as perform biocontrol of fungal pathogens. The…
Descriptors: Plants (Botany), Science Instruction, Science Laboratories, Biotechnology
Riley, Erin; Felse, P. Arthur – Chemical Engineering Education, 2017
Centrifugation is a major unit operation in chemical and biotechnology industries. Here we present a simple, hands-on laboratory experiment to teach the basic principles of centrifugation and to explore the shear effects of centrifugation using bacterial cells as model particles. This experiment provides training in the use of a bench-top…
Descriptors: Laboratory Experiments, Microbiology, Scientific Concepts, Chemistry
Taipa, M. A^ngela; Azevedo, Ana M.; Grilo, Anto´nio L.; Couto, Pedro T.; Ferreira, Filipe A. G.; Fortuna, Ana R. M.; Pinto, Ine^s F.; Santos, Rafael M.; Santos, Susana B. – Journal of Chemical Education, 2015
An integrative laboratory study addressing fundamentals of enzyme catalysis and their application to reactors operation and modeling is presented. Invertase, a ß-fructofuranosidase that catalyses the hydrolysis of sucrose, is used as the model enzyme at optimal conditions (pH 4.5 and 45 °C). The experimental work involves 3 h of laboratory time…
Descriptors: Cooperative Learning, Science Experiments, College Science, Undergraduate Study
Novo, M. Teresa; Casanoves, Marina; Garcia-Vallvé, Santi; Pujadas, Gerard; Mulero, Miquel; Valls, Cristina – Journal of Biological Education, 2016
We present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a…
Descriptors: Foreign Countries, Preservice Teachers, Preservice Teacher Education, Science Education
Mate, Karen; Sim, Alistair; Weidenhofer, Judith; Milward, Liz; Scott, Judith – Biochemistry and Molecular Biology Education, 2013
A blended approach encompassing problem-based learning (PBL) and structured inquiry was used in this laboratory exercise based on the congenital disease Osteogenesis imperfecta (OI), to introduce commonly used techniques in biomolecular analysis within a clinical context. During a series of PBL sessions students were presented with several…
Descriptors: Diseases, Molecular Biology, Science Instruction, Problem Based Learning
Makarevitch, Irina; Kralich, Elizabeth – Biochemistry and Molecular Biology Education, 2011
Open-ended, inquiry-based multiweek laboratory exercises are the key elements to increasing students' understanding and retention of the major biological concepts. Including original research into undergraduate teaching laboratories has also been shown to motivate students and improve their learning. Here, we present a series of original…
Descriptors: Genetics, Laboratory Experiments, Science Activities, Science Course Improvement Projects
Mulimani, V. H.; Dhananjay, K. – Journal of Chemical Education, 2007
This laboratory experiment was designed to demonstrate the application of immobilized galactosidase in food industry to hydrolyze raffinose family oligosaccharides in soymilk. This laboratory experiment was conducted for postgraduate students of biochemistry and developed for graduate and undergraduate students of biochemistry, biotechnology,…
Descriptors: Undergraduate Students, Biotechnology, Biochemistry, Chemical Engineering
Schendel, Marilyn Shimizu – Bioscene, 1999
Describes a freshman biology laboratory project that uses the polymerase chain reaction to introduce students to the interrelationship between biology and chemistry. Students must develop their own experimental protocol, perform calculations introduced in freshman classes, and evaluate group dynamics. (Author/WRM)
Descriptors: Biochemistry, Biology, Biotechnology, Chemistry