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Vimoshini Pragash; Meran Keshawa Ediriweera – Biochemistry and Molecular Biology Education, 2025
The Folch method is a commonly employed, simple biochemical procedure used to extract fat from food items and biological samples in biochemistry laboratories. The iodine number of fat reflects its degree of unsaturation. In the present study, the iodine number of fat extracted using the Folch method from cow milk, goat milk, chicken egg yolk and…
Descriptors: Biochemistry, Science Laboratories, Laboratory Procedures, Science Experiments
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Andrew J. Tonsager; Laurie A. Stargell – Biochemistry and Molecular Biology Education, 2025
CRISPR-Cas9 technology is an established, powerful tool for genome editing through the ability to target specific DNA sequences of interest for introduction of desired genetic modifications. CRISPR-Cas9 is utilized for a variety of purposes, ranging from a research molecular biology tool to treatment for human diseases. Due to its prominence…
Descriptors: Undergraduate Students, Student Research, Scientific Research, Genetics
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Candela, Maria Fernanda; Arenas, Nelson E.; Caicedo, Obradith; Malagon, Andres – Journal of Chemical Education, 2021
The inhibition of porcine pancreatic lipase by the drug orlistat was studied through a series of activities that combine experimental and computational procedures. The enzymatic activity of the lipase was determined by measuring enzyme kinetics and calculating parameters such as K[subscript m] and V[subscript max] from experimental data. Assays of…
Descriptors: Biochemistry, Drug Therapy, Kinetics, Measurement
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Nicola´s Andre´s Saffioti; Carla Angelani; Jose´ Mari´a Delfino; Silvia Ferna´ndez Villamil; Gabriela Elena Go´mez; Irene Mangialavori; Mari´a Florencia Pignataro; Pablo Julio Schwarzbaum – Journal of Chemical Education, 2023
Metabolic Control Analysis (MCA) is a systems biology approach that measures the control of a metabolic step on the overall flux of a metabolic pathway. In undergraduate courses in biochemistry, learning of MCA can be encouraged by experimental activities demonstrating practical applications of the MCA theory. In this work, we present a laboratory…
Descriptors: Biochemistry, Metabolism, College Science, Undergraduate Students
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Odunuga, Odutayo O.; Cheatwood, Nicholas Y.; Mullins, John A.; Nguyen, Samantha K.; Fry, Darrell R.; Harris, Michele R. – Journal of Chemical Education, 2021
This paper describes an undergraduate biochemistry laboratory module consisting of a set of experiments designed around a purification scheme for bovine serum albumin (BSA). Students purify BSA from cow plasma by a combination of salt and acetone precipitation, equilibrium dialysis, ion exchange, and size exclusion chromatography. Students use the…
Descriptors: Undergraduate Students, College Science, Science Instruction, Instructional Design
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Applegate, Kaitlin B.; Cheek, Patrick R.; Inlow, Jennifer K. – Biochemistry and Molecular Biology Education, 2019
Laboratory exercises for undergraduate biochemistry students are described in which changes in sugar content during fermentation of the trendy beverage kombucha are analyzed by three methods: thin layer chromatography, a 3,5-dinitrosalicylic acid assay, and a standard commercial blood glucose meter. Each of the three analyses can be completed in a…
Descriptors: Majors (Students), Chemistry, Biology, College Science
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Silverstein, Todd P.; Kirk, Sarah R.; Meyer, Scott C.; Holman, Karen L. McFarlane – Biochemistry and Molecular Biology Education, 2015
We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure,…
Descriptors: Science Instruction, Science Laboratories, Science Process Skills, Spectroscopy
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Silverberg, Lee J.; Coyle, David J.; Cannon, Kevin C.; Mathers, Robert T.; Richards, Jeffrey A.; Tierney, John – Journal of Chemical Education, 2016
Imines are important in biological chemistry and as intermediates in organic synthesis. An experiment for introductory undergraduate organic chemistry is presented in which benzaldehyde was condensed with "p"-methoxyaniline in toluene to give 4-methoxy-"N"-(phenylmethylene)benzenamine. Water was removed by azeotropic…
Descriptors: Undergraduate Students, Organic Chemistry, Laboratory Experiments, Introductory Courses
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Rosu, Cornelia; Cueto, Rafael; Veillion, Lucas; David, Connie; Laine, Roger A.; Russo, Paul S. – Journal of Chemical Education, 2017
Volatile compounds from polymeric materials such as weatherstripping were identified by solid-phase microextraction (SPME), a solvent-free analytical method, coupled to gas chromatography-mass spectrometry (GC-MS). These compounds, originating from additives and fillers used in weatherstripping processing, were mostly polycyclic aromatic…
Descriptors: Undergraduate Students, High School Students, College Science, Secondary School Science
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Novo, M. Teresa; Casanoves, Marina; Garcia-Vallvé, Santi; Pujadas, Gerard; Mulero, Miquel; Valls, Cristina – Journal of Biological Education, 2016
We present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a…
Descriptors: Foreign Countries, Preservice Teachers, Preservice Teacher Education, Science Education
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Wilson, Karl A.; Tan-Wilson, Anna – Biochemistry and Molecular Biology Education, 2013
Mass spectrometry (MS) has become an important tool in studying biological systems. One application is the identification of proteins and peptides by the matching of peptide and peptide fragment masses to the sequences of proteins in protein sequence databases. Often prior protein separation of complex protein mixtures by 2D-PAGE is needed,…
Descriptors: Spectroscopy, Biochemistry, Science Instruction, Molecular Biology
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Garrett, Teresa A.; Rose, Rebecca L.; Bell, Sidney M. – Biochemistry and Molecular Biology Education, 2013
In this laboratory module, introductory biochemistry students are exposed to two-dimensional [superscript 1]H-nuclear magnetic resonance of glycerophospholipids (GPLs). Working in groups of three, students enzymatically synthesized and purified a variety of 2-acyl lyso GPLs. The structure of the 2-acyl lyso GPL was verified using [superscript…
Descriptors: Diagnostic Tests, Science Laboratories, Biochemistry, College Science
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Cartrette, David P.; Miller, Matthew L. – Journal of Chemical Education, 2013
An innovative first- and second-year laboratory course sequence is described. The goal of the instructional model is to introduce chemistry and biochemistry majors to the process of research participation earlier in their academic training. To achieve that goal, the instructional model incorporates significant hands-on experiences with chemical…
Descriptors: Science Laboratories, College Science, Chemistry, Biochemistry
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Pederson, Dennis M. – Journal of Chemical Education, 1974
Descriptors: Biochemistry, Chemistry, College Science, Demonstrations (Educational)
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Turchi, Sandra; And Others – Journal of Chemical Education, 1989
Describes an undergraduate biochemistry laboratory experiment on enzyme kinetics using the D-amino acid oxidase system and an ammonia electrode. Preparation of an ammonia standard curve, a sample preparation, and inhibition studies are discussed. (YP)
Descriptors: Biochemistry, Chemical Reactions, Chemistry, College Science
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