Bacteria have developed a cell-to-cell communication system called quorum sensing (QS), allowing them to regulate group behavior and synchronize the expression of virulence factors, responsible for increasing their infection capacity and resistance to antimicrobials. Although the control of microbial infections through the inhibition of microbial…

After a time away from the classrooms and laboratories due to the global pandemic, the return to teaching activities during the semester represented a challenge to both teachers and students. Our particular situation in a Microbial Physiology course was the necessity of imparting in shorter time, laboratory practices that usually take longer. This…

Short chain fatty acids (SCFAs) are produced in the gut as a result of microbial action on ingested dietary fiber and have been associated with several health benefits. Herein an undergraduate student experiment that uses gas chromatography equipped with either a flame ionization detector or mass spectrometer for the analysis and quantitation of…

Rhizobacteria that colonize plant roots and promote plant growth are referred to as plant growth-promoting rhizobacteria, and this can stimulate plant growth either indirectly or directly. Volatile organic compounds (VOCs) emitted by rhizobacteria have the capacity to promote plant growth as well as perform biocontrol of fungal pathogens. The…

"Sinorhizobium meliloti" is a bacterium of great agroeconomic importance because of its ability to fix atmospheric nitrogen in symbiotic association with alfalfa ("Medicago sativa") roots, and it is often used in model studies. We investigated the effects of exopolysaccharides (EPSs) in cell-to-cell and cell-to-surface…

The convenient synthesis of dihydroberberine by the reduction of berberine is described as an experiment for an upper-division undergraduate organic chemistry laboratory course. Students obtained up to 74% yield of the desired pure product without the use of chromatographic techniques. The antimicrobial activities of both compounds against…

The MicRoboCop project introduces chemistry students to the concepts and protocols of synthetic biology. MicRoboCop is a biosensor that has been designed for unique applications in forensic chemistry. The lab is carried out in collaboration with an instructor teaching a genetics course. Students in the biology course synthesize biosensors using…

We describe a 2 h introductory laboratory procedure that prepares a novel magnetic antimicrobial activated carbon nanocomposite in which nanoscale sized magnetite and silver particles are incorporated (MACAg). The MACAg nanocomposite has achieved the synergistic properties derived from its components and demonstrated its applicability as an…

During the Jurassic period (201 million to 145 million years ago), termites up to 15 mm long consumed and recycled vegetation and feces. Since then, termites have evolved into some 3,000 identified species, have colonized every continent except Antarctica, and are major contributors to nutrient cycling and vertebrate food webs (Shaw 2014).…

Food spoilage has an enormous economic impact, and microbial food spoilage plays a significant role in food waste and loss; subsequently, an equally significant portion of undergraduate food microbiology instruction should be dedicated to spoilage microbiology. Here, we describe a set of undergraduate microbiology laboratory exercises that focus…

Background: We present an analysis of students' responses to application-based questions on the topic of growth and control of microorganisms, from a questionnaire administered to 348 second and third year students of an Indian university who were enrolled in its undergraduate programs in Biotechnology or Microbiology. We examined aspects of the…

Biochemistry courses in the Department of Molecular Biology at the National University of Río Cuarto, Argentina, are designed for undergraduate students in biology, microbiology, chemistry, agronomy, and veterinary medicine. Microbiology students typically have previous coursework in general, analytical, and organic chemistry. Programmed sequences…

Experimental science activities in primary school enable important cross-curricular learning. In this study, experimental activities on microbiology were carried out by 16 pupils in a Portuguese grade-4 classroom (9-10?years old) and were focused on two problem-questions related to microbiology and health: (1) do your teeth carry microbes? (2) why…

We outline protocols for producing slant-minis (SLINIs) and mini-deeps (MEEPs) and examples of their use in simple microbiology experiments suitable for high school students. The principal benefits of these protocols are decreased cost associated with significantly reduced media use; easier, less expensive disposal of waste; and increased safety…

Cell signaling is an essential cellular process that is performed by all living organisms. Bacteria communicate with each other using a chemical language in a signaling pathway that allows bacteria to evaluate the size of their population, determine when they have reached a critical mass (quorum sensing), and then change their behavior in unison…