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Singh, Anoushka; Miller, Ryan C.; Archuleta, Stephen R.; Kugel, Jennifer F. – Biochemistry and Molecular Biology Education, 2023
Transcription is the critical first step in expressing a gene, during which an RNA polymerase (RNAP) synthesizes an RNA copy of one strand of the DNA that encodes a gene. Here we describe a laboratory experiment that uses a single assay to probe two important steps in transcription: (1) RNAP binding to DNA, and (2) the transcriptional activity of…
Descriptors: Molecular Biology, Science Instruction, Genetics, Teaching Methods
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Li, Chanjuan; Wu, Gaobing; Fan, Xuezhu; Guo, Siqi; Qin, Yuqing; Hu, Yonggang; Ruan, Lifang – Biochemistry and Molecular Biology Education, 2023
This article describes a comprehensive practical laboratory method for developing an enzyme to more easily measure glyphosate levels in solution. Through this article, undergraduate students of biology majors can conduct research experiments in critical fields by utilizing various techniques, such as chemiluminescence (CL) biosensors with…
Descriptors: Biochemistry, Scientific Concepts, Biotechnology, Laboratory Experiments
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Crane, Julia; Page, Shallee T. – Biochemistry and Molecular Biology Education, 2022
We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published…
Descriptors: Undergraduate Students, College Science, Science Instruction, Scientific Research
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Khalid, Habiba; Amin, Farrukh Raza; Chen, Chang – Biochemistry and Molecular Biology Education, 2022
An integrated laboratory experiment was designed for introducing biochemistry students to basic static biochemistry to deepen their understanding on the properties and analysis of biomolecules such as total carbohydrates, lipid, protein, and protein-constituent amino acids. Food represents a very important source of biomolecules of technological…
Descriptors: Laboratory Experiments, Science Experiments, Introductory Courses, Biochemistry
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Suárez Casanova, Víctor Manuel; Shumskaya, Maria – Biochemistry and Molecular Biology Education, 2021
DNA structure has been leveraged in a variety of facets that allow scientists to perform a range of assays, including ones for identification of species, establishing evolutionary relationships between taxa, or even identifying individuals. Here, we present a DNA barcoding method as practical, hands-on approach that connects several experimental…
Descriptors: Genetics, Biochemistry, Science Instruction, Molecular Biology
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Kallarackal, Angy J. – Teaching of Psychology, 2023
Background: The goals of laboratory experiences include developing knowledge base, research skills, and scientific communication abilities. Objective: The aim was to assess an inquiry-based laboratory activity using the model organism "Caenorhabditis elegans" in relation to learning goals. Method: Students in a "Biopsychology"…
Descriptors: Laboratory Experiments, Biology, Psychology, Communication Skills
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Mangis, J. W.; Mansur, T. B.; Kern, K. M.; Schroeder, J. R. – Bioscene: Journal of College Biology Teaching, 2019
Undergraduate research is a valuable tool to demonstrate both the dedication and time required to be a successful biologist. One area of research that has intrigued students over the last several years is cytotoxicity. However, at smaller undergraduate institutions, the time, training, and funding available for these research studies may be…
Descriptors: Undergraduate Students, Student Research, Scientific Research, Cytology
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Large, Delaney N.; Van Doorn, Nathaniel A.; Timmons, Shannon C. – Biochemistry and Molecular Biology Education, 2023
Laboratory courses should cultivate enthusiasm for research and an appreciation for real-world scientific challenges to retain undergraduate students and encourage them to pursue STEM-related careers. Course-based undergraduate research experiences (CURE) have emerged as an inclusive pedagogical model that facilitates laboratory skill development,…
Descriptors: Cancer, Laboratory Experiments, Undergraduate Students, STEM Careers
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Sarisky, Catherine A.; Johann, Timothy W. – Journal of Chemical Education, 2018
A semester-long hypothesis-driven laboratory project for second-semester biochemistry has been developed. Working independently, students propose a hypothesis about the role of one amino acid residue in the active site of 5,10-methenyltetrahydrofolate synthetase (MTHFS). They then test this hypothesis by site-directed mutagenesis of a plasmid…
Descriptors: Biochemistry, Science Instruction, Laboratory Experiments, Course Descriptions
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Gokalp, Sumeyra; Horton, William; Jónsdóttir-Lewis, Elfa B.; Foster, Michelle; Török, Marianna – Biochemistry and Molecular Biology Education, 2018
To facilitate learning advanced instrumental techniques, essential tools for visualizing biomaterials, a simple and versatile laboratory exercise demonstrating the use of Atomic Force Microscopy (AFM) in biomedical applications was developed. In this experiment, the morphology of heat-denatured and amyloid-type aggregates formed from a low-cost…
Descriptors: Biochemistry, Biomedicine, Visualization, Laboratory Training
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Farnham, Kate R.; Dube, Danielle H. – Biochemistry and Molecular Biology Education, 2015
Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically…
Descriptors: Biochemistry, Laboratory Experiments, Student Projects, Research Projects
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Novo, M. Teresa; Casanoves, Marina; Garcia-Vallvé, Santi; Pujadas, Gerard; Mulero, Miquel; Valls, Cristina – Journal of Biological Education, 2016
We present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a…
Descriptors: Foreign Countries, Preservice Teachers, Preservice Teacher Education, Science Education
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Hancock, Dale; Funnell, Alister; Jack, Briony; Johnston, Jill – Biochemistry and Molecular Biology Education, 2010
An experiment is conducted, which in four 3 h laboratory sessions, introduces third year undergraduate Biochemistry students to the technique of real-time PCR in a biological context. The model used is a murine erythroleukemia cell line (MEL cells). These continuously cycling, immature red blood cells, arrested at an early stage in erythropoiesis,…
Descriptors: Undergraduate Students, Genetics, Cytology, Biochemistry
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Bellin, Robert M.; Bruno, Mary K.; Farrow, Melissa A. – Biochemistry and Molecular Biology Education, 2010
We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of "Thermus aquaticus" DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single…
Descriptors: Majors (Students), Undergraduate Students, Science Laboratories, Biochemistry
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Vincent, John B.; Woski, Stephen A. – Journal of Chemical Education, 2005
A laboratory course called cytochrome c that focuses on the theme of biochemical research is presented. The students follow this course by incorporating team-investigation and self-directed experimentation that provides them an opportunity to experience the excitement of research.
Descriptors: Laboratory Experiments, Biochemistry, Science Experiments, Scientific Research
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