Technological Pedagogical Content Knowledge (TPACK) is a model of CALL competence to integrate technology into education. Cultivating attitudes of EFL teachers in light of online instruction and examining its relationship with TPACK seems to be a crucial aspect of current teaching practices. Thus, this correlational study attempted to explore the relationship between Iraqi EFL teachers' attitudes towards online instruction and TPACK components. Sixty teachers of English as a foreign language from Thi-Qar, selected via availability sampling, took part in the study. They responded to two questionnaires, one with 28 items on TPACK and the other containing 16 items on online teaching English. A series of Pearson correlation tests were conducted to examine the seven research hypotheses about the participants' attitudes towards online instruction and technological pedagogical content knowledge components, including CK, PK, TK, TCK, PCK, TPK, and TPACK. The findings demonstrated a range of low to moderate positive associations between instructors' attitudes towards online instruction and TPACK domains. It was indicated that the teachers with positive attitudes were also positive about their perceived TPACK. The present research increases our understanding of TPACK in relation to online teaching in contexts where EFL is practiced.