Mastering correct pronunciation is crucial for effective Spanish language learning and communication. However, many existing language learning resources lack adequate coverage of pronunciation guidance and feedback mechanisms. This study aims to address this gap by developing SpanishBot, an innovative chatbot designed to facilitate the learning of Spanish letter pronunciations and their variations for non-native speakers. Forty-eight participants, unfamiliar with Spanish, were randomly divided into an experimental group using SpanishBot and a control group using independent learning methods. The experimental group interacted with SpanishBot over a 2-month period, which utilized Python programming and the Line instant messaging platform for delivering personalized pronunciation feedback and practice. Instruments included a pre-test and a post-test, as well as the SpanishBot system, which provided real-time feedback on pronunciation accuracy based on audio recordings. Findings revealed a statistically significant difference between the two groups' post-test scores, with the experimental group demonstrating remarkable improvements in Spanish pronunciation proficiency. This study highlights SpanishBot's potential in language education, specifically for Spanish pronunciation training.