Background/purpose: This study aimed to investigate the effectiveness of a computerized instructional module based on artificial intelligence in acquiring scientific concepts and developing critical thinking among seventh-grade female students. Materials/methods: To achieve the study's objectives, an AI-based computerized instructional module was created, and two tests were prepared to measure their performance in acquiring scientific concepts and critical thinking. The study sample consisted of two classes of seventh-grade female students selected conveniently in the first semester of the 2023/2024 academic year, totaling 48 students. One class was randomly chosen as the experimental group of 27 students, taught using the computerized instructional module based on artificial intelligence. In contrast, the other class was the control group, with 21 students taught using the traditional method. Results: The study results showed a statistically significant difference in the acquisition of scientific concepts, both individually and collectively, and the development of critical thinking, both individually and collectively, between the performance of the two study groups in favor of the experimental group who studied using the artificial intelligence-based instructional module. Conclusion: The study recommended the importance of using smart applications in education, especially in the field of science teaching, due to their significant impact on achieving interactivity and practical experience, which contributes to enhancing students' understanding and motivating them to discover more scientific concepts innovatively and enjoyably and developing their critical thinking.