Personal (PSs) and professional (PRs) skills are necessary to any physical education (PE) teacher, potentially having an impact on the quality of teaching and students' engagement in PE. In recent years, the Teachers' Personal and Professional Skills Questionnaire (TPPS-Q), composed of two 5-item dimensions (PSs and PRs), has been developed and tested. This study aims to test the validity of the TPPS-Q in the Czech school context. The analysis of the questionnaire and its structure, which was based on responses from 135 pupils enrolled in the last grades of Czech primary schools, was performed using the software AMOS for structural equation modeling. Results suggest that the structural model is appropriate after removing two items (CFI = 0.981; RMSEA = 0.058; SRMR = 0.041). Internal validity was good for both PSs (Alpha = 0.811; Omega = 0.814) and PRs (Alpha = 0.795; Omega = 0.794). Reliability was also found to be good (PSs: ICC = 0.888; PRs: ICC = 0.760). Additional scores were assessed for convergent and discriminant validity. The final 8-item version is valid and can be used in the Czech school environment.