Describes an instructional experiment that was designed to illustrate the use of periodate oxidation in sugar analysis, and the consequence that the absorbance of mixtures of non-interacting substances is additive. (TW)

Presents a practical exercise that enables students to measure flux control coefficients in a metabolic pathway in vitro, to verify the summation property, and to compare the differences in the distribution of these coefficients in different steady states. Discusses background, materials, methods, and results of the activity. (CW)

Suggests a new experiment to study the genetic material of eukaryotes. It is based on the rapid growth and interest of the research on chromatin. (TW)

Describes two complementary laboratory exercises that use the glass fiber assay to assess receptor specificity and hormone binding affinity in rat liver cytoplasmic extracts. Details the methods, materials and protocol of the experiments. Discusses the basic concepts illustrated and the feasibility of using the experiments at the undergraduate…

Describes three activities, substrate inhibition, product inhibition by fructose and glucose, and gel immobilization of invertase for use with undergraduate biochemistry classes. Discusses materials, methods, and results. Stresses the advantages of practical exercises in undergraduate classes. (CW)

Describes a program to support lectures on analytical centrifugation, and to illustrate the manner in which useful analytical data about macromolecules can be obtained from simple centrifugation studies without the need for mathematical expertise. Discusses the background, methods, calculations, and results involved in this activity. (CW)

Illustrates the scope of experimental investigations for biochemistry education in high school biology and chemistry courses. Gives a brief overview of biochemistry experiments with proteins, enzymes, carbohydrates, lipids, nucleic acids, vitamins, metabolism, electron transport, and photosynthesis including materials, procedures, and outcomes.…

Examines the influence of enzyme and substrate concentrations, pH, temperature, and inhibitors on catalytic activity. Follows the influence of different phosphate concentrations in the growth medium on enzyme activity. Studies regulation of enzyme synthesis by repression. Includes methodology for six experiments. (MVL)

Offers several advantages over other possibilities as the enzyme of choice for a student's first exposure to a purification scheme. Uses equipment and materials normally found in biochemistry laboratories. Incorporates several important biochemical techniques including spectrophotometry, chromatography, centrifugation, and electrophoresis. (MVL)