The need for shifting the expository laboratory instruction style to inquiry-based approaches is widely acknowledged. Problem-based learning (PBL), one of the inquiry-based approaches, advocates students' self-directed learning. The literature indicates that scaffolding students' independent learning is necessary for a PBL environment. In our…

This laboratory exercise is an inquiry-based investigation developed around the core experiment where students, working alone or in groups, each purify and analyze their own prescreened colored proteins using immobilized metal affinity chromatography (IMAC). Here, we present reagents and protocols that allow 12 different proteins to be purified in…

Three undergraduate students functioned as teaching assistants in unit ops lab for their senior project, which included developing a purchased size exclusion chromatography experiment, supervising the running of this experiment, and assisting the junior students with their calculations. They also read the reports, graded them, and commented on…