The cardinal virtues (prudence, justice, fortitude, and temperance) have relevance within the areas of character education and integral or comprehensive formation. In recent years, there has been growing interest and a great deal of literature produced on character education and its measurement. In this paper, we propose a questionnaire (a Likert-type self-report scale of 22 items), the QCV, designed to measure competencies associated with the cardinal virtues. The theoretical foundation of the questionnaire lies in the Aristotelian-Thomistic tradition in dialogue with positive psychology. The paper also offers a review of the literature on the measurement of virtue and the principal instruments currently used to measure the cardinal virtues. The results of a preliminary application of the QCV with a sample of 325 university students indicate that the questionnaire, written in Spanish and conducted in Madrid, has good psychometric properties.