Discusses structure and function of enzymes, design of new enzymes and enzyme substitutes, and enzyme uses in industry, medicine, and wastewater treatment. The latter is a low-cost method which can remove as much as 99 percent of toxic substances found in many industrial wastewater streams. (JN)

Described is a bioluminescent assay for adenosine triphosphate (ATP) and reduced nicotineamide-adenine dinucleotide (NADH) that meets the requirements of an undergraduate biochemistry laboratory course. The 3-hour experiment provides students with experience in bioluminescence and analytical biochemistry yet requires limited instrumentation,…

Enzyme technology has a well-established industrial base, with applications that have survived competition. The most prominent applications of enzymes in biotechnology are examined with an explanation of some theoretical background. Topics include extending an enzyme's useful life, partition and diffusion, industrial uses, and therapeutic uses.…

The primary component(s), impurities, and degradation products of polysorbate 80, nitrate and nitrite salts, and diethylpyrocarbonate (DEPC) are discussed. Safety considerations related to these food additives are also noted. The chick-edema factor which results from an additive in poultry feed is also discussed. (JN)

Describes an experiment which demonstrates changes in ammonia excretion and urine pH that occur in response to metabolic acidosis (induced by ammonium chloride ingestion) or metabolic alkalosis (produced by sodium bicarbonate ingestion). List of materials needed and background information are included. Typical results are provided and discussed.…

Reviews literature on chromatography examining: books, reviews, student experiments; chromatographic systems, techniques, apparatus; detecting and identification of separated zones; preparative chromatography and radiochromatography; and applications related to specific materials (such as acids, alcohols, amino acids, antibiotics, enzymes, dyes,…

Presents designs for atomic models for building small cyclic molecules and demonstrates their use in the construction of beta-lactam antibiotic structures. (MLH)

Evidence suggests that a well-developed lecture script on a topic in medical biochemistry can quickly and easily be converted into an effective slide-tape program that is as educationally effective and well-received as one that is painstakingly tailored to the nature of the medium. (LBH)

Outlines lectures on protein and virus x-ray crystallography given in a graduate biophysics course; also relates recent advances and further references pertaining to protein crystallography. (MLH)

Describes an organic-biological experiment that illustrates an important chemical commercial process and the use of microorganisms as chemical reagents. (MLH)

Presents short questions and answers on biochemical topics, such as respiration, enzymatic reactions, symbiotic relationships, and oxygen consumption of sea animals. (MLH)

Describes an experiment in which the iodonation of aniline reaction is shown to undergo catalysis in solution of sodium lauryl sulfate which forms micelles with negatively charged pseudo surfaces. (MLH)

Describes an advanced undergraduate experiment in which the stepwise stability constants of hi(II)-cysteine chelate are determined. (MLH)

Described is the development of bioelectrochemistry and biorheology research. Stressed is the importance of continued development of interdisciplinary research. (SL)